Chicken IgM ELISA Assay

$475.00

The Chicken IgM ELISA Assay Kit is a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring IgM in serum or plasma of Chickens.

Chicken IgM ELISA Assay

The Chicken IgM ELISA Assay is For Research Use Only

Size: 1×96 wells
Sensitivity: 0.979 ng/ml
Dynamic Range: 3.125 ng/ml – 200 ng/ml
Incubation Time: 70 minutes
Sample Type: Serum, plasma
Sample Size: 100 µL


Assay Principle for Chicken IgM ELISA

The principle of the double antibody sandwich ELISA is represented in Figure 1. In this assay the IgM present in samples reacts with the anti-IgM antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-IgM antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound IgM. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of chicken IgM in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of IgM in the test sample. The quantity of chicken IgM in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.


SPECIMEN COLLECTION AND HANDLING
Blood should be collected by venipuncture. The serum should be separated from the cells after clot formation by centrifugation. For plasma samples, blood should be collected into a container with an anticoagulant and then centrifuged. Care should be taken to minimize hemolysis, excessive hemolysis can impact your results. Assay immediately or aliquot and store samples at -20C. Avoid repeated freeze-thaw cycles.


Related Products

Chicken IgY ELISA Kit
Chicken Serum Amyloid A Assay Kit
Chicken IgA ELISA Assay

Additional Information

Typical Standard Curve


Chicken IgM ELISA Assay

Documents

Product Documents


Product Citations