Certolizumab Pegol mAb-based ELISA
The Certolizumab Pegol mAb-based ELISA is For Research Use Only
Size: 1 x 96 wells
Sensitivity: 2 ng/mL
Dynamic Range: 6 – 200 ng/mL
Incubation Time: 3 hours
Sample Type: Serum, Plasma
Sample Size: 10 µL
Alternative Name: Cimzia
This Certolizumab Pegol mAb-based ELISA is based on Certolizumab pegol-specific mouse monoclonal antibody (catcher Ab, ImmunoGuide clone CY). Standards and diluted samples are incubated in the microtiter plate coated with IG-CY mAb. After incubation, the wells are washed. A biotinylated recombinant human tumor necrosis factor alpha (rhTNF-α) is added and binds to the Fab part of Certolizumab pegol. Following incubation, wells are washed and the horseradish peroxidase (HRP)-conjugated streptavidin is added and binds to the biotinylated rhTNF-α. Following incubation, wells are washed and the bound enzymatic activity is detected by addition of chromogen-substrate. The colour developed is proportional to the amount of Certolizumab pegol in the sample or standard. Results of samples can be determined by using the standard curve. Preincubation of Certolizumab pegol with rhTNF-α inhibited the reaction in a concentration dependent manner. Therefore, the ImmunoGuide Certolizumab pegol ELISA (mAb-based) measures the free form of Certolizumab pegol.Pipette 100 µL of Assay Buffer into each of the wells to be used.
Pipette 50 µL of each 1:500 Diluted Standard, and 1:500 Diluted Samples into the respective wells of the microtiter plate.
Cover the plate with adhesive seal. Shake plate carefully. Incubate 60 min at room temperature (RT, 20-25°C).
Remove adhesive seal. Aspirate or decant the incubation solution. Wash the plate 3 X 300 μL of Diluted Wash Buffer per well. Remove excess solution by tapping the inverted plate on a paper towel.