Bream IGF-I (Pagrus auratus)
The Eagle Bioscience’s Bream IGF-I (Pagrus auratus) is for research use only and not to be used for diagnostic procedures.
IGF-I is produced in E.coli using a patented expression system and the IGF-I is correctly folded and purified by several chromatography steps to yield Receptor Grade IGF-I.
Bream insulin-like growth factor-I (IGF-I) is a single chain 67 amino acid polypeptide that shares structural homology with human IGF-I. It is identical to [Gly1] gilthead sea bream (Sparus aurata) IGF-I and [Gly1] black bream (Acanthopagrus butcheri) IGF-I as glycine replaces the serine in these IGFs. Bream IGF-I can be used for a variety of in vivo and in vitro applications including those investigating the actions of growth hormones.
- Purity: >90 % (as determined by HPLC analysis)
- Quantity: 100 µg
- Molecular Weight: 7379 daltons – confirmed by Mass Spectrometry
- N-terminal sequence analysis of recombinant product: 5 residues >95% single sequence
- Biological Activity: (stimulation of protein synthesis in rat L6 myoblasts) ED50 < 50 ng/ml)
- Endotoxin (LAL): < 0.1 EU/μg
- Storage/Stability: At least 2 years at 2-4°C (lyophilized)
- Appearance: White Powder
- State: Dried from 0.1 M acetic acid under dry nitrogen at a slight vacuum (-25kPa)