Bovine Albumin ELISA Assay
The Bovine Albumin ELISA Assay is For Research Use Only
Size: 1×96 wells
Sensitivity: 50 ng/ml
Dynamic Range: 10 -40,960 ng/ml
Incubation Time: 2.5 hours
Sample Type: Serum, Urine, Biological Fluids
Sample Size: 5 µL
Alternative Name: Cow Albumin ELISA Assay
Sample Preparation and Storage
Dilute the samples with diluent. To exclude matrix effects the dilution factor should be at least 1:5 (e. g. 50 µl sample + 200 µl diluent). A 1:100 dilution is useful for urine samples (e. g. 5 µl urine + 495 µl diluent). If samples generate values outside the standard curve, the dilution factor may be quite varied. Store the undiluted samples at –20 °C.
The determination of albumin is carried out as direct competitive ELISA. Bovine albumin has been pre-coated onto a microplate. During incubation the binding of an enzyme-linked anti-albumin (bovine)-antibody to the wells is inhibited by albumin from samples/standards. After washing away any unbound antibody, a substrate solution is added to the wells and color develops in proportion to the amount of antibody conjugate. The absorption at 450 nm is reverse proportional to the albumin concentration.