Big Endothelin-1 ELISA Assay
Big Endothelin-1 ELISA Assay Developed and Manufactured in Austria by Biomedica
Size: 1×96 wells
Sensitivity: 0.02 pmol/ml
Dynamic Range: 0.1 – 3 pmol/L
Incubation Time: 5.5 hours
Sample Type: serum, plasma
Sample Size: 50 µL
Alternative Names: Big ET-1 ELISA, Human Big Endothelin-1 ELISA, Human Big ET-1 ELISA
For Research Use Only
Values From Apparently Healthy Individuals (n=41):
Serum: Median: 0.09 pmol/l
Each laboratory should establish its own reference range for the samples under investigation.
Do not change sample type during studies.
The Big Endothelin-1 ELISA Assay kit is a sandwich enzyme immunoassay for the quantitative determination of Big Endothelin-1 in human serum and plasma samples. In a first step, standard/control/sample and detection antibody (monoclonal mouse anti- human Big Endothelin-1 antibody) are pipetted into the wells of the microtiter strips, which are pre-coated with polyclonal sheep anti-human Big Endothelin-1 antibody. Big Endothelin-1 present in the standard/control/sample binds to the pre-coated antibody in the well and forms a sandwich with the detection antibody. After a washing step, which removes all non-specifically bound and unbound material, the conjugate (streptavidin-HRP) is pipetted into the wells and reacts with the detection antibody. After another washing step, the substrate (tetramethylbenzidine, TMB) is pipetted into the wells. The enzyme-catalyzed color change of the substrate is directly proportional to the amount of Big Endothelin-1. This color change is detectable with a standard microtiter plate reader. The concentration of Big Endothelin-1 in the sample is determined directly from the dose response curve.
Areas of Interest
- prognostic value in heart failure and acute myocardial infarction
- renal insufficiency
- during and after graft rejection
- pulmonary hypertension and scleroderma
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Learn more about Endothelin at Eagle Biosciences Biomarker Spotlight page dedicated to Endothelin here: EagleBio Spotlight: Endothelin