Bevacizumab mAb-based ELISA Assay


The Bevacizumab mAb-based ELISA Assay is an enzyme immunoassay for the precise analytical determination of free Bevacizumab in serum and plasma samples.  The Bevacizumab (Avastin®) (mAb-based) ELISA Assay Kit is for research use only and not to be used in diagnostic procedures.

Bevacizumab mAb-based ELISA Assay

The Bevacizumab mAb-based ELISA Assay is For Research Use Only

Size: 1×96 wells
Sensitivity: 2 ng/mL
Dynamic Range: 6 – 200 ng/mL
Incubation Time: 2 hours
Sample Type: Serum, Plasma
Sample Size: 10 µL
Alternative Names: Avastin Assay

Controls Included

Assay Principle

This ELISA is based on Bevacizumab-specific mouse monoclonal antibody (catcher Ab, ImmunoGuide clone MAY-2B5). Standards and diluted samples are incubated in the microtiter plate coated with IG-MAY-2B5 mAb. After incubation, the wells are washed. A horseradish peroxidase (HRP)-conjugated anti-human IgG monoclonal antibody is added and binds to the Fc part of Bevacizumab. Following incubation, wells are washed and the bound enzymatic activity is detected by addition of chromogen-substrate. The colour developed is proportional to the amount of Bevacizumab in the sample or standard. Results of samples can be determined by using the standard curve. Binding of Bevacizumab to the solid phase, pre-coated with MAY-2B5, is inhibited by human VEGF-A in a concentration dependent manner. Therefore, the ImmunoGuide Bevacizumab ELISA (mAb-Based) measures the free form of Bevacizumab.

The kit is shipped at ambient temperature and should be stored at 2-8°C. Keep away from heat or direct sun light. The microtiter strips are stable up to the expiry date of the kit in the broken, but tightly closed bag when stored at 2–8°C.

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Additional Information

Assay Background

The drug Bevacizumab (trade name Avastin® ) is a recombinant human IgG1::k monoclonal antibody specific for all human vascular endothelial growth factor-A (VEGF-A) isoforms and it has been approved by the FDA as a first-line treatment for metastatic colorectal cancer in combination with chemotherapy. Furthermore, VEGF is implicated in intraocular neovascularization associated with diabetic retinopathy and age-related macular degeneration.

The specificity of this test system is achieved by using a monoclonal antibody named “MAY-2B5” for the coating of the microtiter plate. This antibody is specific for Bevacizumab only and does not cross react with other VEGF catchers.

Typical Standard Curve

Bevacizumab mAb-based ELISA Assay


Product Manual


Please note: All documents above are for reference use only and should not be used in place of the documents included with this physical product. If digital copies are needed, please contact us.



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