AT1R IgG2 Antibody ELISA
The AT1R IgG2 Antibody ELISA is For Research Use Only
Sensitivity: 2.5 U/mL
Dynamic Range: 2.5 – 40 U/ml
Incubation Time: 3.5 hours
Sample Type: Serum, Plasma
Sample Size: 100 µL
Alternative Name: Anti Angiotensin II Receptor 1 (AT1R) IgG2 Antibody ELISA Assay Kit
Sample Preparation and Storage
Dilute the samples with diluent using 1:100 dilution (eg. 5 µl serum or plasma + 495 µl diluent). If samples generate values outside the standard curve, the dilution factor may be quite varied. Store the undiluted samples at room temperature for 48 hours, 2-8°C 4- days, and long-term storage for up to 12 months at –20 °C. Avoid repeated freeze-thaw cycles.
The Eagle Biosciences Human Anti Angiotensin Receptor 1 IgG2 ELISA Assay Kit is an antibody screening test. Angiotensin II Receptor has been pre-coated onto a microtiter plate. During the first incubation the anti-Angiotensin II receptor 1-IgG2-Antibodies of the samples are immobilized on the plate. The autoantibodies are detected with a POD labeled anti-human IgG antibody. In the following enzymatic substrate reaction the intensity of the color correlates with the concentration and/ or avidity of anti-Angiotensin II receptor 1-IgG2-Antibody.