AT1R IgG2 Antibody ELISA


The AT1R IgG2 Antibody ELISA Kit is designed for the determination of IgG2-Antibodies against the Angiotensin II receptor subtype I in serum and plasma.  The Anti Angiotensin Receptor 1 (ATR1)IgG2 Antibody ELISA Assay Kit is for research use only and not to be used in diagnostic procedures.

SKU: AG231-K01 Categories: , ,

AT1R IgG2 Antibody ELISA

The AT1R IgG2 Antibody ELISA is For Research Use Only

Sensitivity: 2.5 U/mL
Dynamic Range: 2.5 – 40 U/ml
Incubation Time: 3.5 hours
Sample Type: Serum, Plasma
Sample Size: 100 µL
Alternative Name: Anti Angiotensin II Receptor 1 (AT1R) IgG2 Antibody ELISA Assay Kit
Controls Included

Sample Preparation and Storage
Dilute the samples with diluent using 1:100 dilution (eg. 5 µl serum or plasma + 495 µl diluent). If samples generate values outside the standard curve, the dilution factor may be quite varied. Store the undiluted samples at room temperature for 48 hours, 2-8°C 4- days, and long-term storage for up to 12 months at –20 °C. Avoid repeated freeze-thaw cycles.

Assay Principle

The Eagle Biosciences Human Anti Angiotensin Receptor 1 IgG2 ELISA Assay Kit is an antibody screening test. Angiotensin II Receptor has been pre-coated onto a microtiter plate. During the first incubation the anti-Angiotensin II receptor 1-IgG2-Antibodies of the samples are immobilized on the plate. The autoantibodies are detected with a POD labeled anti-human IgG antibody. In the following enzymatic substrate reaction the intensity of the color correlates with the concentration and/ or avidity of anti-Angiotensin II receptor 1-IgG2-Antibody.

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Additional Information

Assay Background

Angiotensin II is a peptide hormone which causes vasoconstriction, increased blood pressure, and release of aldosterone from the adrenal cortex. It occupies an important role in the ReninAngiotensin-System (RAS). Angiotensin II mediates the effects through G-Protein-coupled receptors, the Angiotensin receptors. The occurrence of autoantibodies against AT1 receptor is associated with an increased risk of an immunologic rejection after an organ transplantation. The presence of AT1 autoantibodies correlate with the existence and course of Scleroderma.

Assay Procedure

  1. Prepare all reagents and samples as directed in the previous section.
  2. Pipette 100 µl of diluted samples, standards, controls or diluent (as blank) into the wells.
  3. Seal wells with adhesive strip and incubate for 2 hours at 2-8°C temperature.
  4. Aspirate fluid from wells and wash three times with 300 µl wash buffer. After the last wash, invert the plate and tap on a clean paper towel.
  5. Dispense 100 μl of diluted HRP conjugate into each well.
  6. Seal wells with adhesive strip and incubate for 1 hour (with shaking) at room temperature.
  7. Repeat the wash as in step 4.
  8. Dispense 100 µl of TMB substrate solution into each well.
  9. Incubate for 25 minutes at room temperature in the dark.
  10. Add 100 µl of stop solution to each well.
  11. Determine the absorbance within 30 minutes at 450 nm. A reference wavelength of 620 nm/690 nm is recommended.

Typical Standard Curve

Package Inserts

Please note: All documents above are for reference use only and should not be used in place of the documents included with this physical product. If digital copies are needed, please contact us.

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