Antioxidant Capacity Potential Assay


The Eagle Biosciences Antioxidant Capacity Potential Assay kit is intended for the quantitative determination of adduct antioxidant capacity in biological samples as well as well food and beverage samples by enzyme linked immunoassay (ELISA). The Antioxidant Capacity Potential Assay kit is for research use only and not to be used in diagnostic procedures.

SKU: PAC39-K01 Categories: , ,

Antioxidant Capacity Potential Assay

The Antioxidant Capacity Potential Assay is For Research Use Only

Size: 1×96 wells
Sensitivity: 0.063 mM
Dynamic Range: 21.9 – 4378 µmol/L (cupric ion reducing power)
Incubation Time: 3 minutes
Sample Type: Human and Animal Serum, Food, Beverages
Sample Size: 200 µL

Preparation of samples
For serum samples, fresh frozen samples are recommended because some antioxidants such as vitamin C, uric acid and coenzyme Q10 are unstable. For other samples types such as beverages and food, see section “Assay Examples”, and dilute with distilled water.

Assay Background

Oxidative stress plays on important role in various diseases and aging. The control of oxidative stress is expected to be useful to prevent diseases and aging.  Oxidative stress is caused by the imbalance between reactive oxygen species (ROS) and antioxidant defense system.  For accurate assessment of oxidative stress, measurement of ROS, oxidative damage and antioxidant activity may be essential. The Potential Antioxidant Capacity Assay can detect not only hydrophilic antioxidants such as Vitamin C and glutathione, but also can detect hydrophobic antioxidants such as Vitamin E. This kit is applicable for assessment of total antioxidants of serum, foods and beverage samples.

Related Products

CUPRAC Food and Beverage Antioxidant Assay
Total Antioxidant Power Microplate Assay
Glutathione Total Assay Kit

Additional Information

Assay Principle

Samples are mixed with Cu++ Solution. Cu++ are reduced by antioxidants to form Cu+. Reduced Cu+ react with Chromatic Solution (Bathocuproine), and can be detected by absorbance at wavelength 480 to 490 nm. Antioxidant capacity can be calculated from the Cu+ formed.

Assay Procedure

  1. Prepare 6 levels of standards by diluting 2mM uric acid.
  2. Please prepare plastic test tubes for 6 levels of standards and each sample. Pour 390 µL of Sample Diluent, and add 10 µL of standards or diluted samples.
  3. Pour 200 µL of mixture to Micro titer plate. Use 200 µL of Sample Diluent for blank well.
  4. Read absorbance at 490 nm (as READ1).
  5. Add 50 µL of Cu++solution to each well, mix gently, and incubate at room temperature for 3 minutes.
  6. Add 50 µL of Stop solution, mix gently, and read absorbance at 490 nm (as READ2).
  7. Please draw standard curves by plotting the difference of absorbance readings (READ2 – READ1) as vertical axis, and concentration of uric acid standards (mM) as horizontal axis. Calculate the corresponding uric acid concentration of samples. Multiply corresponding uric acid concentration (mM) of samples by 2189, to estimate antioxidant power (µmol/L).


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