Anti-Prostaglandin H Synthase-1 Monoclonal Antibody
The Anti-Prostaglandin H Synthase-1 Monoclonal Antibody is For Research Use Only
Product Number: PHS00-A100
Aliquot: 100 µl
Storage: -20° C
Product Developed and Manufactured in the USA
Immunoglobulin Type: Monoclonal Mouse IgG
Specificity: This antibody was developed against ovine PGHS-1 and specifically reacts with ovine PGHS-1 at 1:100 or higher dilution.
Applications: For Western blotting and Immunohistochemistry using alkaline phosphatase or horseradish peroxide, a dilution of 1:1000 is recommended. With a more sensitive detection system such as enhanced chemiluminescence or ELISA, the antibody may be diluted further.
Contents per Vial: 100 mL of ascetic fluid containing 0.1% sodium azide.
Storage: Stable for several weeks at 4°C or several months at -20°C. Avoid repeated freeze/thaw cycles.
DeWitt, D.L., et al., Concentrations of PGH Synthase and PGI2 Synthase in the Endothelium and Smooth Muscle of Bovine Aorta. J. Clin. Invest. 72: 1882 (1983).
DeWitt, D.L., et al., Orientation of the Active Site and Antigenic Determinants of Prostaglandin Endoperoxide (PGH) Synthase in the Endoplasmic Reticulum. J. Biol. Chem. 256: 10375 (1981).
Pagels, W.R., et al., Immunochemical Evidence for the Involvement of Prostaglandin Endoperoxide Synthase in Hydroperoxide-Dependent Oxidations by Ram Seminal Vesicles. J. Biol. Chem. 258: 6517 (1983).
Smith, W.L., and Rollins, T.E., Characteristics of Rabbit Anti-PGH Synthase Antibodies and Use in Immunochemistry. Methods in Enzymology 86: 213 (1982).
DeWitt, D.L., et al., Monoclonal Antibodies Against PGH Synthase: An Immunoradiometric Assay for Quantitating the Enzyme. Methods in Enzymology 86: 229 (1982).