Anti-Muscarinic Cholinergic Receptor (AChR) 1 (M1) Antibody ELISA


Muscarinic cholinergic receptors (mAChRs) are acetylcholine receptors that form G proteinreceptor complexes in the cell membranes of certain neurons and other cells. The CellTrend anti-muscarinic cholinergic receptor 1 (M1)-Antibody EIA is designed for the determination of antibodies against the muscarinic cholinergic receptor 1 (M1) in serum.

Anti-Muscarinic Cholinergic Receptor (AChR) 1 (M1) Antibody ELISA

For Research Use Only

Size: 1×96 wells
Sensitivity: Cut-off
Dynamic Range: 2.5 – 40 U/ml
Incubation Time: 3.5 hours
Sample Type: Serum
Sample Size: 100 µL

Additional Information

Assay Principle

The CellTrend muscarinic cholinergic receptor 1 (M1) ELISA assay kit is an antibody screening test. M1 receptor has been pre-coated onto a microtiter plate. During the first incubation the anti-muscarinic cholinergic recetor 1-antibodies of the samples are immobilized on the plate. The autoantibodies are detected with an HRP labeled anti-human IgG antibody. In the following enzymatic substrate reaction, the intensity of the color correlates with the concentration and/or avidity of anti-muscarinic cholinergic receptor 1 antibodies.

Assay Procedure

It is recommended that all samples and standards be assayed in duplicates.

1. Prepare all reagents and samples as directed above.
2. Pipette 100 µl of dilution samples, standards, control or diluent DIL SPE (as blank) into well.
3. Seal wells with adhesive strip and incubate for 2 hours at 4°C temperature.
4. Aspirate fluid from wells and wash three times with 300 µl wash buffer. After the last wash, invert the plate and tap on a clean paper towel.
5. Dispense 100 µl of diluted HRP conjugate into each well.
6. Seal wells with adhesive strip and incubate for 1 hour (with shaking) at room temperature.
7. Repeat the wash as in step 4.
8. Dispense 100 µl of the TMB substrate SUBS TMB solution into each well.
9. Incubate for 20 minutes at room temperature in the dark.
10. Add 100 µl of stop solution SOLN STOP to each well.
11. Determine the absorbance within 30 minutes at 450 nm. A reference wavelength of 620 nm/690 nm is recommended.

Typical Standard Curve


Product Manual