AChR M5 Antibody ELISA


The AChR M5 Antibody ELISA is designed for the determination of antibodies against the muscarinic cholinergic receptor 5 (M5) in serum. Muscarinic cholinergic receptors (mAChRs) are acetylcholine receptors that form G proteinreceptor complexes in the cell membranes of certain neurons and other cells.

AChR M5 Antibody ELISA

The AChR M5 Antibody ELISA is For Research Use Only

Sensitivity: Cut-off
Dynamic Range: 2.5 – 40 U/ml
Incubation Time: 3.5 hours
Sample Type: Serum
Sample Size: 100 µL
Alternative Name: Anti-Muscarinic Cholinergic Receptor (AChR) 5 (M5) Antibody ELISA Assay Kit
Controls Included

REAGENTS: Preparation and Storage
This test kit must be stored at 2 – 8 °C upon receipt. For the expiration date of the kit refer to the label on the kit box. All components are stable until this expiration date. Prior to use allow all reagents to come to room temperature. If crystals have formed, mix gently until the crystals have completely dissolved. Reagents from different kit lot numbers should not be combined or interchanged.

Assay Principle

The Eagle Biosciences muscarinic cholinergic receptor 5 (M5) ELISA assay kit is an antibody screening test. M5 receptor has been pre-coated onto a microtiter plate. During the first incubation the anti-muscarinic cholinergic recetor 5-antibodies of the samples are immobilized on the plate. The autoantibodies are detected with an HRP labeled anti-human IgG antibody. In the following enzymatic substrate reaction, the intensity of the colour correlates with the concentration and/or avidity of anti-muscarinic cholinergic receptor 5 antibodies.

Related Products

AChR M2 Antibody ELISA
AChR M3 Antibody ELISA
AChR M4 Antibody ELISA

Additional Information

Assay Procedure

It is recommended that all samples and standards be assayed in duplicates.

  1. Prepare all reagents and samples as directed above.
  2. Pipette 100 µl of dilution samples, standards, control or diluent DIL SPE (as blank) into well.
  3. Seal wells with adhesive strip and incubate for 2 hours at 4°C temperature.
  4. Aspirate fluid from wells and wash three times with 300 µl wash buffer. After the last wash, invert the plate and tap on a clean paper towel.
  5. Dispense 100 µl of diluted HRP conjugate into each well.
  6. Seal wells with adhesive strip and incubate for 1 hour (with shaking) at room temperature.
  7. Repeat the wash as in step 4.
  8. Dispense 100 µl of the TMB substrate SUBS TMB solution into each well.
  9. Incubate for 20 minutes at room temperature in the dark.
  10. Add 100 µl of stop solution SOLN STOP to each well.
  11. Determine the absorbance within 30 minutes at 450 nm. A reference wavelength of 620 nm/690 nm is recommended.

Typical Standard Curve

AChR M5 Antibody ELISA

Package Inserts

Please note: All documents above are for reference use only and should not be used in place of the documents included with this physical product. If digital copies are needed, please contact us.

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