Anti Endothelin Receptor A IgG4 Antibody ELISA Assay Kit

$1,610.00

The Eagle Biosciences Human Anti Endothelin Receptor A IgG4 Antibody ELISA Assay Kit is designed for the determination of antibodies (IgG4) against the Endothelin receptor subtype A in serum and plasma. The Eagle Biosciences Anti Endothelin Receptor A IgG4 Antibody ELISA Assay Kit is for research use only and not to be used in diagnostic procedures.

Anti Endothelin Receptor A IgG4 Antibody ELISA Assay Kit

For Research Use Only

Size: 1×96 wells
Sensitivity: 2.5 U/ml
Dynamic Range: 2.5 – 40 U/ml
Incubation Time: 3.5 hours
Sample Type: Serum, Plasma
Sample Size: 100 µL

Additional Information

Assay Background


Endothelins (ET) are 21-amino acid vasoconstricting peptides produced primarily in the endothelium having a key role in vascular homeostasis. It mediates the effects through G-Protein-coupled receptors, the Endothelin receptors. There are two key receptor types, ETA and ETB. ETA receptors are found in the smooth muscle tissue of blood vessels where they increase vasoconstriction by ET-1.

The Eagle Biosciences Human Anti Endothelin Receptor A IgG4 Antibody ELISA Assay Kit is designed for the determination of antibodies (IgG4) against the Endothelin receptor subtype A in serum and plasma.

Assay Principle


The Eagle Biosciences Human Anti Endothelin Receptor A IgG4 Antibody ELISA Assay Kit is an antibody screening test. Endothelin- receptor A has been pre-coated onto a microtiter plate. During the first incubation the anti-Endothelin receptor A-Antibodies of the samples are immobilized on the plate. The autoantibodies are detected with a POD labelled anti-human IgG4 antibody. In the following enzymatic substrate reaction the intensity of the color correlates with the concentration and/ or avidity of anti-Endothelin receptor A-antibody.

Assay Procedure


  1. Prepare all reagents and samples as directed in the previous section.
  2. Pipette 100 µl of diluted samples, standards, controls or diluent (as blank) into the wells.
  3. Seal wells with adhesive strip and incubate for 2 hours at 2-8°C temperature.
  4. Aspirate fluid from wells and wash three times with 300 µl wash buffer. After the last wash, invert the plate and tap on a clean paper towel.
  5. Dispense 100 µl of diluted HRP conjugate into each well.
  6. Seal wells with adhesive strip and incubate for 1 hour (with shaking) at room temperature.
  7. Repeat the wash as in step 4.
  8. Dispense 100 µl of TMB substrate solution into each well.
  9. Incubate for 20 minutes at room temperature in the dark.
  10. Add 100 µl of stop solution to each well.
  11. Determine the absorbance within 30 minutes at 450 nm. A reference wavelength of 620 nm/690 nm is recommended.

Manual

Product Manual


Publications

Citations


  1. Gabriela Riemekasten, Aurélie Philippe, Melanie Näther, et al. Invovment of functional autoantibodies against vascular receptors in systemic sclerosis. Ann Rheum Dis 2011 Mar; 70(3): 530-536
  2. Hiemann NE, Meyer R, Wellnhofer E, Schoenemann C, Heidecke H, Lachmann N, Hetzer R, Dragun D. Non-HLA Antibodies Targeting Vascular Receptors Enhance Alloimmune Response and Microvasculopathy After Heart Transplantation. Transplantation. 2012 Oct 2