Anti-Cetuximab (ERBITUX) ELISA Assay Kit
The Anti-Cetuximab (ERBITUX) ELISA Assay Kit is For Research Use Only
Size: 12 x 8 wells
Sensitivity: +/-
Incubation Time: 2 hours 20 minutes
Sample Type: Serum, Plasma
Sample Size: 20 µL
Alternative Name: Erbitux
Controls Included
Assay Background
Cetuximab is an epidermal growth factor receptor binding FAB. Cetuximab is composed of the Fv (variable; antigen-binding) regions of the 225 murine EGFr monoclonal antibody specific for the N-terminal portion of human EGFr with human IgG1 heavy and kappa light chain constant (framework) regions. Cetuximab binds to the epidermal growth factor receptor (EGFr) on both normal and tumor cells. EGFr is over-expressed in many colorectal cancers. Cetuximab competitively inhibits the binding of epidermal growth factor (EGF) and TGF alpha, thereby reducing their effects on cell growth and metastatic spread.
Assay Principle
Solid phase enzyme-linked immunosorbent assay {ELISA) based on the sandwich principle. Controls and samples {serum or plasma) are incubated in the microtiter plate coated with the drug cetuximab. After incubation, the wells are washed. Then, horse radish peroxidase {HRP) conjugated probe is added and binds to cetuximab antibodies captured by the drug cetuximab on the surface of the wells. Following incubation wells are washed and the bound enzymatic activity is detected by addition of chromogen-substrate. Finally, the reaction is terminated with an acidic stop solution. The color developed is proportional to the amount of cetuximab antibodies in the sample or controls. The results can be evaluated with using cut-off value.
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