Cetuximab mAb-Based ELISA Assay


The Cetuximab mAb-Based ELISA Assay is an enzyme immunoassay for the specific and precise determination of free Cetuximab in serum and plasma. The Eagle Biosciences Cetuximab mAb-based ELISA Assay Kit is for research use only and not to be used in diagnostic procedures.

Cetuximab mAb-Based ELISA Assay

The Cetuximab mAb-Based ELISA Assay is For Research Use Only

Size: 1 x 96 wells
Sensitivity: 2 ng/mL
Dynamic Range: 6 -200 ng/mL
Incubation Time: < 2 hours
Sample Type:  Serum, Plasma
Sample Size: 10 µL
Alternative Name: Erbitux

Assay Principle

The Cetuximab ELISA is based on Cetuximab-specific mouse monoclonal antibody (catcher Ab, ImmunoGuide clone IG-FB7D9). Diluted standards and diluted samples are incubated in the microtiter plate coated with IG-FB7D9 mAb. After incubation, the wells are washed. A horseradish peroxidase (HRP)-conjugated anti-human IgG monoclonal antibody is added and binds to the Fc part of Cetuximab. Following incubation, wells are washed and the bound enzymatic activity is detected by addition of chromogen-substrate. The colour developed is proportional to the amount of Cetuximab in the sample or standard. Results of samples can be determined by using the standard curve. Binding of Cetuximab to the solid phase, pre-coated with IG-FB7D9, is inhibited by recombinant human epidermal growth factor receptor (rh-EGFR) protein in a concentration dependent manner. Therefore, the ImmunoGuide Cetuximab ELISA (mAb-Based) measures the free form of Cetuximab.

Storage and Stability of the Kit
The Cetuximab ELISA is shipped at ambient temperature and should be stored at 2-8°C. Keep away from heat or direct sun light. The microtiter strips are stable up to the expiry date of the kit in the broken, but tightly closed bag when stored at 2–8°C.
The usual precautions for venipuncture should be observed. It is important to preserve the chemical integrity of a blood specimen from the moment it is collected until it is assayed. Do not use grossly hemolytic, icteric or grossly lipemic specimens. Samples appearing turbid should be centrifuged before testing to remove any particulate material.

Related Products

Cetuximab (Erbitux) ELISA Kit
Anti-Cetuximab (Erbitux) ELISA Assay
Ranibizumab mAb-based Assay Kit

Additional Information

Assay Background

The drug Cetuximab (trade name Erbitux®) is a human-mouse chimeric immunoglobulin (Ig) G1k monoclonal antibody (mAb), selectively directed against the epidermal growth factor receptor (EGFR), also known as HER1 or ErbB1. Since its approval by Food and Drug Administration for cancer treatment in 2004, Cetuximab became widely used in the treatment of colorectal and head and neck cancers. The ImmunoGuide Cetuximab ELISA  (mAb-based) kit can be efficiently used for measuring free Cetuximab levels in serum and plasma.

Typical Standard Curve

Cetuximab mAb-Based ELISA Assay


Product Manual


Please note: All documents above are for reference use only and should not be used in place of the documents included with this physical product. If digital copies are needed, please contact us.



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