Anti-Certolizumab (CIMZIA) Quantitative ELISA Assay Kit
The Anti-Certolizumab (CIMZIA) Quantitative ELISA Assay Kit is For Research Use Only
Size: 12 x 8 wells
Sensitivity: 5 ng/mL
Standard Range: 0 – 160 ng/mL
Incubation Time: 2 hours 20 minutes
Sample Type: Serum, Plasma
Sample Size: 5 µL
Alternative Name: Cimzia
Controls Included
Assay Background
Certolizumab pegol is a pegylated monoclonal antibody against the tumor necrosis factor- alpha (TNF-alpha). It is formed with a humanized Fab fragment of 50 kDa, from an IgG1 isotype, fused to a 40 kDa polyethylene glycol moiety replacing the Fe antibody region. The absence of the Fe region was ideated to prevent complement fixation and antibody- mediated cytotoxicity as well as to markedly increase its half-life. Certolizumab does not require glycosylation for active function and hence, its production is significantly more affordable when compared to other existing TNF-alpha therapies as it can be done directly in bacterial hosts such as E.coli.
Assay Principle
Solid phase enzyme-linked immunosorbent assay (ELISA) based on the sandwich principle. Standards and samples (serum or plasma) are incubated in the microtiter plate coated with the drug Certolizumab. After incubation, the wells are washed. Then, horse radish peroxidase (HRP) conjugated probe is added and binds to Certolizumab antibodies captured by the drug Certolizumab on the surface of the wells. Following incubation wells are washed and the bound enzymatic activity is detected by addition of tetramethylbenzidine (TMB) chromogen substrate. Finally, the reaction is terminated with an acidic stop solution. The color developed is proportional to the amount of Certolizumab antibodies in the sample or standard. Results of samples can be determined directly using the standard curve.
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