AChR M3 Antibody ELISA


This anti-muscarinic cholinergic receptor 3 (M3)-antibody ELISA is designed for the determination of antibodies against the muscarinic cholinergic receptor 3 (M3) in serum. The Eagle Biosciences Anti-Muscarinic Cholinergic Receptor 3 (M3) Antibody ELISA Assay Kit is for research use only and not to be used in diagnostic procedures.

AChR M3 Antibody ELISA

The AChR M3 Antibody ELISA is For Research Use Only

Sensitivity: Cut-off
Dynamic Range: 2.5 – 40 U/ml
Incubation Time: 3.5 hours
Sample Type: Serum
Sample Size: 100 µL
Alternative Name: Anti-Muscarinic Cholinergic Receptor 3 (M3) Antibody ELISA Assay
Controls Included

Assay Background

Muscarinic cholinergic receptors, or mAChRs, are acetylcholine receptors that form G protein-receptor complexes in the cell membranes of certain neurons and other cells. Autoantibodies against M3 AChR are significantly elevated in patients suffering from chronic fatigue syndrome (CFS) / myalgic encephalomyelitis (ME). Muscarinic acetylcholine receptor 3 (M3) autoantibodies are present in a subset of 20-30% of chronic fatigue syndrome patients.

The Eagle Biosciences anti-muscarinic cholinergic receptor 3 (M3)-antibody EIA is designed for the determination of antibodies against the muscarinic cholinergic receptor 3 (M3) in serum.

Related Products

Anti-Muscarinic Cholinergic Receptor (AChR) 4 (M4) Antibody ELISA Assay
Anti-Muscarinic Cholinergic Receptor (AChR) 5 (M5) Antibody ELISA Assay Kit

Additional Information

Assay Principle

The muscarinic cholinergic receptor 3 (M3)-EIA is an antibody screening test. M3 receptor has been pre-coated onto a microtiter plate. During the first incubation the anti-muscarinic cholinergic receptor 3 antibodies of the samples are immobilised on the plate. The autoantibodies are detected with a POD labeled anti-human IgG antibody. In the following enzymatic substrate reaction the intensity of the colour correlates with the concentration and/ or avidity of anti-muscarinic cholinergic receptor 3-antibody.

Typical Standard Curve

Package Inserts

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