20-HETE ELISA Assay Kit
The 20-HETE ELISA Assay Kit is For Research Use Only
Size: 1×96 wells
Sensitivity: 0.01 ng/ml
Dynamic Range: 0.01 – 1000 ng/ml
Incubation Time: 2.5 hours
Sample Type: Serum, Plasma, Tissue, Biological Fluids
Sample Size: 100 µl
Alternative Names: 20-hydroxyeicosatetraenoic acid
The specificity of the 20-HETE ELISA Assay kit was investigated using authentic 20-HETE and a panel of fatty acids which, based on their structure, might be anticipated to compete with 20-HETE for binding to antibodies for 20-HETE. Anti-20-HETE did not cross-react with 14, 15- and 11,12-DHETs, PGE2 and showed almost no cross-reactivity even with structurally extremely similar arachidonic acid (AA), linoleic acid and linolenic acid as shown in the competitive ELISA analysis. Considering the only difference between 20-HETE and AA is an oxygen molecule, the specificity of the Eagle Biosciences 20-HETE ELISA is a surprise.
Human essential and salt-sensitive hypertensions were related to differential AA metabolism by cytochrome P450 (CYP) 4A which has AA-ω-hydroxylase (20-HETE synthesis) activity. Increased circulating insulin inhibits 20-HETE synthesis in obese hypertensive subjects.
Recently, CYP4F2 genetic variants, which increased urinary 20-HETE secretion, were found to be correlated with the risk for hypertension in a Chinese population. The 20-Hete ELISA Assay kit can be used for the determination of 20-HETE in serum, plasma, cells, and tissues following proper isolation and purification.