Clone M-3 Mouse Melanoma Expressing PhiYFP
The Clone M-3 Mouse Melanoma Expressing PhiYFP is For Research Use Only
A bright yellow fluorescent cell line has been established by stable transfection with a plasmid coding for PhiYFP. The M3 mouse melanoma (clone of Cloudman S91-M3) cell line constitutes an ideal model for in vivo experiments. Melanoma-like tumors in different colors can be induced in syngeneic mice by subcutaneous injection of M3-JRed
or M3-PhiYellow cells. After several days tumor formation becomes evident.
Specifications
Cell Line: M3 cells (clone of Cloudman S91-M3 melanoma) expressing PhiYFP
Cells/vial: approximately 5 x 105
Subculturing: 1:3
Medium Renewal: 2 to 3 times per week
Growth Medium: DMEM (1 g/L D-Glucose, 3,7 g/L NaHCO3) + 10 % fetal calf serum (FCS), 2 mM L-Alanyl-LGlutamine, 1 % Non Essential Amino Acids (NEA)
Freeze Medium: Complete growth medium + 10% FCS, 10% DMSO
Shipping: Frozen
Storage Recommendation: Liquid Nitrogen
Biosafety Level: 1
INFORMATION:
Organism: Mus musculus (mouse)
Source: Organ: skin; Disease: melanoma
Gender: male
Tumorigenic: yes
Growth properties: adherent
Morphology: epithelial
Conditions: 37 °C, 5 % CO2
Plasmid: pPhiYFP-C (Evrogen, Moscow, Russia)
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