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Mediagnost along with Eagle Biosciences, Inc. has released an improved test for human Adiponectin in serum and plasma samples*. This Total Adiponectin ELISA Assay Kit has been upgraded with the following features;

• is extremely sensitive (less than 0.3 ng/ml)
• is fast: incubation time a total of 1 hour and 45 minutes
• Single Standards with 2, 10, 30, 70, 100 ng/ml human Adiponectin are provided in the Kit
• 2 Control Sera are provided for quality control purposes according GLP
• is calibrated with native Adiponectin
• uses high affinity monoclonal antibodies against human Adiponectin
• Microtiter plates are separately breakapart
• Available for human, mouse and rat samples

For more information click here

Read IFU Here 

*The Adiponectin ELISA is for Research Use Only

We have been very busy these past few months gathering a bunch of new products to offer our great and loyal customers! Some of these new products have been added to our ever-growing Bone Metabolism Assays.

Research on the effects of various cancers and abnormalities in the skeletal system are on the rise and we are excited to start offering more tests! 

Soluble Semaphorin 4D ELISA: 

For the direct determination of soluble Semaphorin 4D in human plasma samples. 

Semaphorin 4D (SEMA4D or CD100) is a member of a family of trans membranes and secreted proteins that regulates key cellular functions and is involved in cell-cell communication. SEMA4D participates in numerous physiological processes such as axon guidance, immune regulation, angiogenesis, tumor progression, and bone metabolism. SEMA4D has emerged to a novel therapeutic target in cancer and in bone diseases. 

Bioactive Sclerostin ELISA:

For the quantitative determination of bioactive sclerostin in human serum, EDTA plasma, and citrate plasma.

Sclerostin is nearly exclusively produced in osteocytes. Mutations in the Sclerostin (SOST) gene can cause sclerosteosis and van Buchem disease which are bone dysplasia disorders characterized by progressive skeletal overgrowth. Sclerostin levels are altered in response to hormonal stimuli or due to pathophysiological conditions. Sclerostin concentrations are increased in disorders such as hypoparathyroidism, Paget’s disease, multiple myeloma and in cancer induced bone diseases.

OsteomiR (Coming Soon!) 

For the quantification of selected microRNA biomarker candidates for fracture-risk in postmenopausal and diabetic osteoporosis in human serum samples.

MicroRNAs are small non-coding RNAs that regulate gene expression through RNA interference and therefore contribute to the regulation of correct cell and tissue function. MicroRNA secretion (contained in exosomes or microvesicles) has been observed in many different cell types, and is believed to serve as an endocrine pathway for cell-to-cell communication. Therefore, the analysis of circulating microRNAs in serum, urine or saliva can be used to obtain valuable information about tissue physiology and pathophysiology. Circulating microRNAs are a novel class of relevant serum biomarkers.

Keep an eye out for all the other great new products we are releasing in the next few months!

Looking for a specific ELISA? Email us what you’re looking for and we can try to make it happen!

Eagle Biosciences Vitamin D ELISA Kit has been used once again in breakthrough research! 

Background: Influenza continues to cause significant morbidity and mortality each year. Vaccination is the primary prevention; however, its effectiveness may be limited even among young, healthy adults. Vitamin D deficiency is highly prevalent and may be associated with poor vaccine immunogenicity and an increased risk for respiratory infections.

Methods: We conducted a retrospective cross-sectional study among young, healthy military personnel to evaluate the associations between 25(OH)D levels with post-influenza vaccination antibody titers (seroprotection defined as a titer of ≥1:40 post-vaccination) and healthcare encounters for respiratory infections during the 2009-2010 influenza season. 25(OH)D levels were analyzed as continuous and categorical [normal (>30 ng/ml), insufficient (20-30 ng/ml), and deficient (<20 ng/ml)] variables. Separate univariate and multivariable logistic regression models were utilized to determine the associations between 25(OH)D levels with antibody responses and respiratory conditions adjusting for possible confounders.

Results: A total of 437 subjects were evaluated. Most participants were young adults (91% were 18-39 years of age), 50% were male, and 56% resided in the southern U.S. Overall, 152 (35%) were vitamin D deficient, 167 (38%) insufficient, and 118 (27%) had normal 25(OH)D levels. There were no demographic differences by 25(OH)3 category. Only 224 (51%) demonstrated a seroprotective anti-influenza post-vaccination titer, which did not vary by categorical 25(OH)D levels [vitamin D deficient vs. normal: OR 1.10 (0.68-1.78) and insufficient vs. normal: OR 1.25 (0.78-2.01)] or continuous vitamin D levels [OR 0.98 (0.84-1.15)]. There were no associations with respiratory diagnoses between the vitamin D groups.

Conclusion: Vitamin D insufficiency and deficiency were highly prevalent despite evaluating a young, healthy adult population. There were no significant associations between 25(OH)D levels and post-vaccination antibody titers or respiratory infections. Strategies for improving influenza vaccine responses are needed since only one-half of vaccinees demonstrated seroprotective anti-influenza titers.

Reference: Rachel Lee, MD¹, Seunghyun Won, PhD^2,3, Christian Hansen, BS¹ and Nancy Crum-Cianflone, MD, MPH^2,4,5, (1)Operational Infectious Diseases, Naval Health Res. Ctr., San Diego, CA, (2)Infectious Disease Clinical Research Program, Department of Preventive Medicine and Biostatistics, Uniformed Services University of the Health Sciences, Bethesda, MD, (3)Henry M. Jackson Foundation for the Advancement of Military Medicine, Bethesda, MD, (4)Scripps Mercy Hospital, San Diego, CA, (5)Naval Medical Center San Diego, San Diego, CA

Eagle Biosciences, Inc. has teamed up once again with Immundiagnostik to bring it’s customers a series of zonulin ELISA’s. These ELISA’s are used for the quantitative determination of zonulin in serum or stool samples. 

Zonulin is a novel human protein analogue to the zonula occludens toxin derived from Vibrio cholerae which participates in tight junctions between cells of the wall of the digestive tract. Zonulin binds to a specific receptor on the surface of intestinal epithelia and triggers a cascade of biochemical events which induces tight junction disassembly and a subsequent permeability increase of the intestinal epithelia, allowing some substances to pass through and activate immune reactions.

Dr. Fasano and his co-workers found out that the zonulin-zonulin-receptor-system is
more activated in celiac disease and type 1 diabetes mellitus patients. Patients with
active celiac disease showed higher levels of zonulin and anti-zonulin antibodies
compared to non-celiac patients and patients in remission, who were on a glutenfree
diet. 

Concerning the autoimmune type 1 diabetes, in experiments with rats it could be
demonstrated that elevated zonulin levels as well as increased intestinal permeability
precede a type 1 diabetes disease. Conversely, type 1 diabetes could be prevented
by inhibition of zonulin in animal experiments.

In addition, it was reported that many people who suffer from celiac disease also suffer
from other autoimmune disorders. It is suggested that increased levels of zonulin
are a contributing factor to the development of celiac disease and other autoimmune
disorders such as insulin dependent diabetes, multiple sclerosis and rheumatoid
arthritis.

Serum and Stool Zonulin ELISA’s are available now! 

References: 

1. Fasano, A, T Not, W Wang, S Uzzau, I Berti, A Tommasini, and S E Goldblum. 2000.
“Zonulin, a Newly Discovered Modulator of Intestinal Permeability, and Its Expression
in Coeliac Disease.” Lancet 355 (9214) (April 29): 1518–9. doi:10.1016/S0140-
6736(00)02169-3. 

2. Wang, W, S Uzzau, S E Goldblum, and A Fasano. 2000. “Human Zonulin, a Potential
Modulator of Intestinal Tight Junctions.” Journal of Cell Science 113 Pt 24 (December):
4435–40. 

3. Fasano, A. 2001. “Intestinal Zonulin: Open Sesame!” Gut 49 (2) (August): 159–62. 

4. Freemark, Michael, and Lynne L Levitsky. 2003. “Screening for Celiac Disease in
Children with Type 1 Diabetes: Two Views of the Controversy.” Diabetes Care 26 (6)
(June): 1932–9.
Manual IDK® Zonulin ELISA
24 

5. Lazzarotto, Francesca, Daniela Basso, Mario Plebani, Alessandro Moscon, Renato
Zanchetta, and Corrado Betterle. 2003. “Celiac Disease and Type 1 Diabetes.” Diabetes
Care 26 (1) (January): 248–9. 

6. Watts, Tammara, Irene Berti, Anna Sapone, Tania Gerarduzzi, Tarcisio Not, Ronald
Zielke, and Alessio Fasano. 2005. “Role of the Intestinal Tight Junction Modulator
Zonulin in the Pathogenesis of Type I Diabetes in BB Diabetic-Prone Rats.” Proceedings
of the National Academy of Sciences of the United States of America 102 (8)
(February 22): 2916–21. doi:10.1073/pnas.0500178102. 

7. De Magistris, Maria Teresa. 2006. “Zonula Occludens Toxin as a New Promising
Adjuvant for Mucosal Vaccines.” Vaccine 24 Suppl 2 (April 12): S2–60–1. 

8. Sapone, Anna, Laura de Magistris, Michelle Pietzak, Maria G Clemente, Amit Tripathi,
Francesco Cucca, Rosanna Lampis, et al. 2006. “Zonulin Upregulation Is Associated
with Increased Gut Permeability in Subjects with Type 1 Diabetes and Their
Relatives.” Diabetes 55 (5) (May 1): 1443–9. doi:55/5/1443 [pii]. 

9. Thomas, Karen E, Anna Sapone, Alessio Fasano, and Stefanie N Vogel. 2006. “Gliadin
Stimulation of Murine Macrophage Inflammatory Gene Expression and Intestinal
Permeability Are MyD88-Dependent: Role of the Innate Immune Response
in Celiac Disease.” Journal of Immunology (Baltimore, Md. : 1950) 176 (4) (February
15): 2512–21.

In a recent study released by AroCell, the novel proliferation biomarker, Thymadine Kinase 1, is proving to provide more information to researchers studying more than hematological malignancies. With the help of AroCells TK 210 ELISA, there are reports that this test can complement the PSA-related biomarkers that are used to help diagnose prostate cancer.

“The main objective of this study is to determine if TK 210 ELISA can complement the PSA-ralated biomarkers leading to a higher specificity and sensitivity in the diagnosis of prostate cancer.” the objective states in newly released research poster. AroCell TK 210 ELISA May Complement Pro PSA and the Prostate Health Index in Differentiating Non-Cancerous from Cancerous Conditions in Prostate Disease (Jagarlamudi, KK et al.).

“The results indicate that the AroCell TK 210 kit can aid in differentiating the non-cancerous group from the confirmed PCa group in urology patients with PSA values between 2 to 10 µg/L.” the poster continues, “Further clinical studies are needed to establish the role of TK 210 ELISA as a complement to pro PSA and PHI, which could be a valuable tool in prostate cancer diagnosis.”

What Is It?

This Eagle Biosciences High Sensitivity Glucagon ELISA Assay Kit is used for quantitative determination of glucagon in plasma, serum and culture medium supernatant. The kit is characterized by its sensitive quantification and high specificity. In addition, it has no influence by other components in samples.

How Does It Work?

This ELISA kit for determination of glucagon is based on a sandwich enzyme immunoassay with two monoclonal antibodies. Standards or samples, and HRP labeled antibodies are added to the wells of plate coated with antibodies against glucagon. During the incubation antibody – antigen – labeled antibody complex is formed on the surface of the wells. After the incubation and rinsing out excess labeled antibody, HRP enzyme activity is finally determined by 3,3’,5,5’-Tetramethylbenzidine (TMB) and the concentration of glucagon is calculated.

What Is It’s Specifity?

This Eagle Biosciences High Sensitivity Glucagon ELISA Assay Kit has high specificity to glucagon, and shows no significant cross reactivity to Glicentin, Oxyntomodulin, GLP-1 and GLP-2.

For more information or purchasing of this product, visit here