Cetuximab mAb-Based ELISA Assay
The Cetuximab mAb-Based ELISA Assay is For Research Use Only
Size: 1 x 96 wells
Sensitivity: 2 ng/mL
Dynamic Range: 6 -200 ng/mL
Incubation Time: < 2 hours
Sample Type: Serum, Plasma
Sample Size: 10 µL
Alternative Name: Erbitux
Assay Principle
The Cetuximab ELISA is based on Cetuximab-specific mouse monoclonal antibody (catcher Ab, ImmunoGuide clone IG-FB7D9). Diluted standards and diluted samples are incubated in the microtiter plate coated with IG-FB7D9 mAb. After incubation, the wells are washed. A horseradish peroxidase (HRP)-conjugated anti-human IgG monoclonal antibody is added and binds to the Fc part of Cetuximab. Following incubation, wells are washed and the bound enzymatic activity is detected by addition of chromogen-substrate. The colour developed is proportional to the amount of Cetuximab in the sample or standard. Results of samples can be determined by using the standard curve. Binding of Cetuximab to the solid phase, pre-coated with IG-FB7D9, is inhibited by recombinant human epidermal growth factor receptor (rh-EGFR) protein in a concentration dependent manner. Therefore, the ImmunoGuide Cetuximab ELISA (mAb-Based) measures the free form of Cetuximab.
Storage and Stability of the Kit
The Cetuximab ELISA is shipped at ambient temperature and should be stored at 2-8°C. Keep away from heat or direct sun light. The microtiter strips are stable up to the expiry date of the kit in the broken, but tightly closed bag when stored at 2–8°C.
The usual precautions for venipuncture should be observed. It is important to preserve the chemical integrity of a blood specimen from the moment it is collected until it is assayed. Do not use grossly hemolytic, icteric or grossly lipemic specimens. Samples appearing turbid should be centrifuged before testing to remove any particulate material.
