Anti-CCP ELISA Assay Kit
Anti-CCP ELISA Assay Kit Developed and Manufactured by Medipan
Size: 12 x 8 wells
Sensitivity: 0.4 U/mL
Incubation Time: 1 hour 45 minutes
Sample Type: Serum
Sample Size: 100 µL
Alternative Names: Cyclic Citrullinated Peptide
For Research Use Only
Assay Background
Rheumatoid Arthritis (RA) is most often diagnosed by the measurement of rheumatoid factors (RF). The rheumatoid factor is an antibody directed against the Fc-region of IgG. It appears mainly as IgM antibody but also as IgA or IgG subgroup. Rheumatoid factors are present in sera of 70 – 80 % of patients suffering from rheumatoid arthritis. But it is not specific for RA since it will also be found in healthy persons. The incidence depends on the age: for young people it is 1 – 4 %, for older persons it may reach up to 25 %. The advantage of CCP antibodies is a higher sensitivity and specificity for the diagnosis of rheumatoid arthritis in comparison to the rheumatoid factors alone. Anti-CCP is often found at a very early state of the disease and it has a high predictive value for development of the disease.
Assay Principle
The ELISA (Enzyme Linked Immunosorbent Assay) is an immunoassay for the determination of specific antibodies. The strips of the microtiter plate are coated with test-specific antigens. If antibodies are present in the patient´s sample, they bind to the antigens. A secondary antibody conjugated with the enzyme peroxidase detects the generated immune complex. A colorless substrate is converted into the colored product. The signal intensity of the reaction product is proportional to the antibody activity in the sample. After stopping the signal intensity of the reaction product is measured photometrically.
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