Anti-dsDNA ELISA Assay Kit
Anti-dsDNA ELISA Assay Kit is for research use only.
Size: 1 × 96 wells
Sensitivity: 0.135 IU/mL
Dynamic Range: 12.5 – 200 IU/mL
Sample Size: 10 µ
Incubation Time: 1 hour 45 minutes
Sample Type: Serum, Plasma
Alternative Names: Anti dsDNA ELISA, Human Anti-dsDNA ELISA
Controls Included
Assay Background
Anti dsDNA IgG test is used as a biomarker of Systemic Lupus Erythematosus (SLE) and for SLE different diseases. Besides the determination of high titers of antinuclear antibody (ANA), the determination of autoantibodies against dsDNA is one of the ACR criteria (American College of Rheumatology) for the diagnosis of Systemic Lupus Erythematosus (SLE). The determination of the concentration of antibodies can be used to monitor treatment success and predict possible attacks of the disease (SLE).
Assay Principle for Anti-dsDNA ELISA
Anti-dsDNA IgG ELISA Assay Kit is based on the binding of serum or plasma IgG antibodies on dsDNA coated on the microplates. In the first step the antibodies in calibrators, controls or prediluted patient samples bind into the inner surface of the wells. After an incubation the microplate is washed with a wash buffer for removing non-reactive serum components. In the second step an anti human IgG horseradish peroxidase conjugated solution recognizes the IgG class antibodies bound to the immobilized dsDNA antigens. After an incubation any excess of enzyme conjugate, which is not specifically bound, is washed away with the wash buffer. Then a chromogenic substrate solution containing TMB is dispensed into the wells. After 15 minutes of incubation the color development is stopped by adding the stop solution. The solution color changes into yellow. The amount of color is directly proportional to the concentration of the Anti dsDNA IgG antibodies present in the original sample. The concentration of the anti dsDNA IgG antibodies in the sample are calculated through a calibration curve.
