Anti-dsDNA ELISA Assay Kit
Anti-dsDNA ELISA Assay Kit Developed and Manufactured by Medipan
Size: 1×96 wells
Sensitivity: 1 IU/ml
Dynamic Range: 1 – 300 IU/ml
Incubation Time: 2.5 hours
Sample Type: Serum, Plasma
Alternative Names: Anti dsDNA ELISA, Human Anti-dsDNA ELISA
Sample Size: 10 µL
For Research Use Only
Testing 102 non-selected sera from blood donors a specificity of 99% was determined in Anti-dsDNA.
Assay Principle for Anti-dsDNA ELISA
The Anti-dsDNA ELISA Assay is an enzyme immunoassay for the quantitative determination of IgG antibodies to dsDNA. The antibodies of the calibrators, control and diluted patient samples react with dsDNA immobilized on the solid phase of microtiter plates. Highly purified dsDNA coated on the microtiter plate guarantees the specific binding of dsDNA IgG antibodies of the specimen under investigation. Following an incubation period of 60 min at room temperature (RT), unbound sample components are removed by a wash step. The bound IgG antibodies react specifically with anti-human-IgG conjugated to horseradish peroxidase (HRP). Within the incubation period of 30 min at RT, excessive conjugate is separated from the solid-phase immune complexes by the following wash step. HRP converts the colorless substrate solution of 3,3’,5,5’-tetramethyl¬benzidine (TMB) added into a blue product. The enzyme reaction is stopped by dispensing an acidic solution into the wells after 15 minutes at room temperature turning the solution from blue to yew. The optical density (OD) of the solution at 450 nm is directly proportional to the amount of specific antibodies bound. The standard curve is established by plotting the antibody concentrations of the calibrators (x-axis) and their corresponding OD values (y-axis) measured. The antibody concentration of the specimen is directly read off the standard curve. Alternatively, results can be calculated by a semi-quantitative method too using calibrator 2 as cut-off calibrator.