Protein A ELISA Assay Kit
The Protein A ELISA Assay Kit is For Research Use Only
Size: 1 × 96 wells
Sensitivity: 5.12 pg/mL
Dynamic Range: 5.12 – 500 pg/mL
Incubation Time: 2 hours 15 minutes
Sample Type: Biological Products
Sample Size: 100 μL
Controls Not Included
Assay Principle
This kit uses the double-antibody sandwich method to coat the 96-well microtiter plate with capture antibody to prepare the solid phase antibody, add the standards and detection samples, and then add the horseradish peroxidase (HRP)-labeled microtiter antibody to form a sandwich conjugate of solid phase antibody-Protein A-microtiter antibody. After the reaction, wash the plate and then add the substrate for color reaction. The substrate converts from colorless to blue under the catalysis of HRP, and converts to yellow finally under the action of stop solution. Determine the absorbance values (OD values) at 450 nm and 630 nm, with 630 nm as the correction wavelength. OD values are positively correlated with Protein A content in the samples.
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