N8-Acetyl-Spermidine ELISA Assay Kit
The N8-Acetyl-Spermidine ELISA Assay Kit is For Research Use Only
Sizes: 1 x 96 wells
Sensitivity: 0.5 nM
Dynamic Range: 2.4 – 93.75 nM
Sample Size: 20 uL
Incubation Time: Overnight
Sample Type: Plasma, Cell culture supernatant
Controls Included
Assay Background
Polyamines are molecules derived from ornithine (Orn) (i.e., putrescine, spermine, spermidine) or arginine (Arg) (i.e., agmatine). While ornithine decarboxylase is responsible for ornithine degradation and spermidine production, cSAT and nSAT transform spermidine to N8-acetylspermidine (N8-AcSpdn). These molecules are involved in many critical processes such as cell proliferation, nucleic acid synthesis, and cytoprotection from oxidative stress. Several studies have evidenced that polyamine metabolism is frequently increased in cancer which can fuel tumor progression and favor immune escape4. Elevated levels of polyamines in sera of cancer patients have been demonstrated to correlate with poor prognosis.
Assay Principle
Enzyme Immunoassay for the quantitative determination of N8-Acetyl-Spermidine (N8-actSp) in plasma samples. After precipitation and derivatization N8-Acetyl-Spermidine is quantitatively determined by ELISA. The competitive ELISA uses the microtiter plate format. The antigen is bound to the solid phase of the microtiter plate. The processed standards, controls and samples and the solid phase bound analyte compete for a fixed number of antiserum binding sites. When the system is in equilibrium, free antigen and free antigen-antiserum complexes are removed by washing. The antibody bound to the solid phase is detected by an anti-rabbit IgG-peroxidase conjugate using TMB as a substrate. The reaction is monitored at 450 nm. Quantification of unknown samples is achieved by comparing their absorbance with a reference curve prepared with known standards.
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