N1-Acetyl-Spermidine ELISA Assay Kit

$1,185.00

The N1-Acetyl-Spermidine ELISA Assay Kit is a competitive ELISA kit for the quantitative measurement of N1-Acetyl-Spermidine (N1-AcSpdn) in plasma samples. The N1-Acetyl-Spermidine ELISA Assay Kit is for research use only and not to be used for diagnostic procedures.

SKU: IS-I-2400R Categories: , ,

N1-Acetyl-Spermidine ELISA Assay Kit

The N1-Acetyl-Spermidine ELISA Assay Kit is For Research Use Only

Sizes: 1 x 96 wells
Sensitivity: 3.5 nM
Dynamic Range: 8 – 312.5 nM
Sample Size: 50 uL
Incubation Time: Overnight
Sample Type: Plasma, Cell culture supernatant

Controls Included


Assay Background

Polyamines are molecules derived from ornithine (Orn) (i.e., putrescine, spermine, spermidine) or arginine (Arg) (i.e., agmatine). While ornithine decarboxylase is responsible for ornithine degradation and spermidine production, cSAT and nSAT transform spermidine to N1-acetylspermidine (N1-AcSpdn). These molecules are involved in many critical processes such as cell proliferation, nucleic acid synthesis, and cytoprotection from oxidative stress. Several studies have evidenced that polyamine metabolism is frequently increased in cancer which can fuel tumor progression and favor immune escape. Elevated levels of polyamines in sera of cancer patients have been demonstrated to correlate with poor prognosis.

Assay Principle

Enzyme Immunoassay for the quantitative determination of N1-Acetyl-Spermidine (N1-actSp) in plasma samples. After precipitation and derivatization N1-Acetyl-Spermidine is quantitatively determined by ELISA. The competitive ELISA uses the microtiter plate format. The antigen is bound to the solid phase of the microtiter plate. The processed standards, controls and samples and the solid phase bound analyte compete for a fixed number of antiserum binding sites. When the system is in equilibrium, free antigen and free antigen-antiserum complexes are removed by washing. The antibody bound to the solid phase is detected by an anti-rabbit IgG-peroxidase conjugate using TMB as a substrate. The reaction is monitored at 450 nm. Quantification of unknown samples is achieved by comparing their absorbance with a reference curve prepared with known standards.


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Documents

Product Manual


Please note: All documents above are for reference use only and should not be used in place of the documents included with this physical product. If digital copies are needed, please contact us.

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