L-Ornithine ELISA Assay Kit
The L-Ornithine ELISA Assay Kit Developed and Manufactured in France by ImmuSmol
Size: 1 x 96 wells
Standard Range: 20 – 781.25 uM
Sensitivity: 8.5 uM
Incubation Time: 3 hours
Sample Type: Plasma
Sample Size: 10 µL
Assay Principle
After extraction and derivatization Ornithine is quantitatively determined by ELISA. The competitive ELISA uses the microtiter plate format. The antigen is bound to the solid phase of the microtiter plate. The processed standards, controls and samples and the solid phase bound analyte compete for a fixed number of antiserum binding sites. When the system is in equilibrium, free antigen and free antigen-antiserum complexes are removed by washing. The antibody bound to the solid phase is detected by an anti-rabbit IgG-peroxidase conjugate using TMB as a substrate. The reaction is monitored at 450 nm. Quantification of unknown samples is achieved by comparing their absorbance with a reference curve prepared with known standards.
Storage and Stability
Store the unopened reagents at 2 – 8°C until expiration date. Do not use components beyond the expiry date indicated on the kit labels. Once opened the reagents are stable for 1 month when stored at 2 – 8°C. Once the resealable pouch has been opened, care should be taken to close it tightly with desiccant again.
