High Sensitive C-Reactive Protein FAST ELISA Assay
The Ultra High Sensitive C-Reactive Protein FAST ELISA Assay is For Research Use Only
Size: 1×96 wells
Sensitivity: 10 ng/mL
Dynamic Range: 100 – 10,000 ng/mL
Incubation Time: 1 hour
Sample Type: Serum
Sample Size: 10 µL
Alternative Name: hs-CRP, Ultrasensitive CRP, C-Reactive Protein
Controls Included
Assay Background
C-reactive protein (CRP) is a pentameric acute phase reactant that is synthesized by the liver. It’s production is controlled primarily by interleukin-6. The serum CRP concentration may increase by up to 1000-fold with infection, trauma, surgery, and other acute inflammatory events. Chronic inflammatory disorders such as auto-immune diseases and malignancy can produce persistent high levels of serum CRP.
Traditionally, CRP has been used clinically for the diagnosis and monitoring of auto-immune and infectious disorders. Recent studies have shown that chronic inflammation is an important component in the development and progression of atherosclerosis. As a result, increased serum CRP concentration are positively associated with the risk of future coronary events.
Assay Principle for Ultra High Sensitive C-Reactive Protein FAST ELISA Assay
The principle of the following enzyme immunoassay test follows the typical two-step capture or ‘sandwich’ type assay. The assay makes use of two highly specific monoclonal antibodies: A monoclonal antibody specific for CRP is immobilized onto the microplate and another monoclonal antibody specific for a different region of CRP is conjugated to horse radish peroxidase (HRP). CRP from the sample and standards are allowed to bind to the plate, washed and subsequently incubated with the HRP conjugate. After a second washing step, the enzyme substrate is added. The enzymatic reaction is terminated by addition of the stopping solution. The absorbance is measured on a microtiter plate reader. The intensity of the color formed by the enzymatic reaction is directly proportional to the concentration of CRP in the sample. A set of standards is used to plot a standard curve from which the amount of CRP in samples and controls can be directly treated.
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