Hamster PLBL2 ELISA Assay
The Hamster PLBL2 ELISA Assay is For Research Use Only
Size: 1×96 wells
Dynamic Range: 20 ng/mL – 0.3125 ng/ml
Incubation Time: 170 minutes
Sample Type: Tissue Culture Fluids
Sample Size: 100 μL
Alternative Names: Hamster CHO PLBL2 ELISA, Hamster (CHO) Phospholipase B-Like 2 (PLBL2) ELISA Kit
Assay Principle
The principle of the double antibody sandwich ELISA is represented in Figure 1. In this assay the PLBL2 present in samples reacts with the anti-PLBL2 antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, the Detection Antibody, biotin conjugated anti-PLBL2 is added and complexes are formed. Following a wash step, the horseradish peroxidase (HRP) conjugated Streptavidin is added and complexes are formed. Following another washing step, the complexes are assayed by the addition of a chromo-genic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of PLBL2 in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of PLBL2 in the test sample. The quantity of PLBL2 can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.
Specimen Collection and Handling
Blood should be collected by venipuncture. The serum should be separated from the cells after clot formation by centrifugation. For plasma samples, blood should be collected into a container with an anticoagulant and then centrifuged. Care should be taken to minimize hemolysis, excessive hemolysis can impact your results. Assay immediately or aliquot and store samples at -20C. Avoid repeated freeze-thaw cycles.
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