Technical Spotlight Archives

Technical Spotlight

EagleBio Highlights CLEIA Kits

Eagle Biosciences proudly offers a broad range of products in our extensive catalog. In addition to the well known ELISA format, we offer several assay kits in a Chemiluminescent Enzyme Immunoassay (CLEIA) format.

ELISA vs. CLEIA

ELISAs use antibodies to detect the presence of a protein in a liquid sample. In the most common form, antigens are applied to a stable surface (usually a microtiter plate). Then the corresponding antibody, linked with an enzyme, is added to form a complex. Finally, the enzyme’s substrate is added, which produces a measurable reaction, usually in the form of a color change.

CLEIAs also utilize antibody-enzyme reactions, but offer a higher level of sensitivity due use of chemiluminescence rather than colorimetric absorbance or fluorescence. Chemiluminescence allows detection of trace amounts of analytes because samples typically have little to no background interference. These reactions are also easily measured using a photomultiplier or photodiode. These types of kits are increasing in popularity due to their increased sensitivity, specificity, reliability, and reproducibility.

Our available CLEIA/CLIA kits

GFAP (Glial Fibrillary Acidic Protein) CLIA Assay Kit
MedFrontier FGF23 (Human Intact FGF23) CLEIA ELISA Assay

Technical Spotlight

Technical Spotlight: DotDiver

by Eagle Biosciences

DotDiver2.0 is a fully automatic processor for Dot/LINE immunoassays manufactured by German biotech Generic Assays.

DotDiver2.0 processes and evaluates assay kits with minimal user intervention. It can perform up to 24 tests at one time. It features bar code identification for test strips and cartridges. DotDiver provides digitized results, and it is LIMS ready or can be set up to print on paper. All you have to do is load the tests and press start, no need to monitor while it’s running. The system requires minimal maintenance and takes up little space.

DotDiver2.0 assays are available for:

Celiac disease / Crohn‘s disease
Pernicious anemia
Neuropathies
Anti-phospholipid syndrome
Systemic lupus erythematosus
Vasculitis
Nephritis
Myositis
Scleroderma
Autoimmune hepatitis

For more information, see the DotDiver 2.0 Immunoassay Processor product page.

Technical Spotlight

Technical Spotlight: MHC Molecules

by Eagle Biosciences

What are MHC molecules?

Major histocompatibility complex (MHC) molecules play an important role in the acquired immune system of vertebrates. MHC molecules present peptides derived from pathogens on the cell surface so that T-cells can determine the appropriate immune response. The MHC also plays a role in mediating leukocyte interactions, determining compatibility for organ transplants, and determining autoimmune disease susceptibility. In humans, the MHC complex is also known as the human leukocyte antigen (HLA) complex.

The peptide-MHC (pMHC) interaction to cognate T-cell receptors (TcR) occurs rapidly and at low affinity. Tetramerizing these molecules on a streptavidin scaffold engages multiple TcRs expressed on a given T cell, which stabilizes the reaction and allows for specific T cell staining. pMHC monomers and tetramers can also be used for purification and manipulation of T cells.

Research Applications

MHC monomers and tetramers can be used for selection and proliferation of specific T cells, allowing researchers to isolate specific viral or tumor related antigens. These antigens can be reintroduced to augment the immune system. They are also used in organ transplant research to help reduce the risk of graft-versus-host disease. Additionally, researchers in cancer immunotherapy and vaccine development are exploring various MHC multimer applications to further their fields.

What Eagle Biosciences Offers

We offer a wide range of pMHC monomers and tetramers through our partner, ImmunAware, including easYmer MHC tetramer kits. All of the MHC molecules in are catalog are biotinylated, meaning all of the pMHC monomers can be tetramerized with the laboratory’s choice of strepatavidin label.

View all of our monomer, tetramer, and easYmer kits here.

For more information or assistance finding a specific product, please contact us.

Technical Spotlight

Technical Spotlight: Metal Enhanced Fluorescence Assays

by Eagle Biosciences

What is Metal Enhanced Fluorescence?

Metal Enhanced Fluorescence (MEF) is based on the reaction of excitation light with electrons of metal nano-structures to generate strong electromagnetic fields, also known as LSPs (localized surfaced plasmons). When LSPs are bound to the surface of the metal nano-structure, it creates a plasmonic structure, which can enhance the signal of the phosphores more than 100 times.

About our FluoBolt™ MEF Assays

Eagle Biosciences proudly offers a line of Metal Enhanced Fluorescence Immunoassays from our partner, Fianostics. These products from the FluoBolt™ line are highly sensitive, single-step assays that require no enzyme substrates. FluoBolt™ MEFs are completely compatible with the 96-well ELISA format and provide a stable signal over time.

We currently offer the following FluoBolt™ MEF Immunoassays:

With many more to come!