Vitamin K1 HPLC Assay
The Vitamin K1 HPLC Assay is For Research Use Only
Size: 100 tests
Incubation Time: 10 minutes
Sample Type: Plasma, Serum
Sample Size: 1 mL
Reference Values (Normal): 0.5 – 5 ng/mL
It is suggested that each laboratory establish its own normal ranges.
Vitamin K was first discovered by Dam in 1929. He fed chicken with ether extracted food and recognized deadly bleeding. Chicken supplemented with the ether extract normalized the symptoms. The hypothetic coagulation factor was called vitamin K. Vitamin K1 (phylloquinone) is synthesized by green plants and taken up by nutrition, whereas vitamin K2 (menaquinone) is produced by bacteria in the gut.
Vitamin K works as cofactor of an oxygen dependent carboxylase, which carboxylates specific glutamic acid residues in g-position. Because of that modification, blood clotting factors can be activated by cleavage. In bone metabolism the g-carboxylation enables osteocalcin to bind to hydroxyapatite of the bone matrix. Vitamin K can be stored only for a short time, whereby deficiency occur after a view days. A lack of vitamin K mainly manifests in clotting disorders. Beside this, disorders in bone metabolism caused by a deficient modification of osteocalcin have been reported.
The Eagle Biosciences Vitamin K1 HPLC Assay kit makes it possible to determine the vitamin in an easy, fast and precise method. The kit includes all reagents for the preparation and separation of the samples with exception of the columns (IC2400rp) and the controls (IC2400ko). Both can be supplied by Eagle Biosciences. Beside the complete test kit it is possible to order all components separately. Please request our single component price list.