Vedolizumab Antibodies ELISA Assay
Vedolizumab Antibodies ELISA Assay for Research Use Only
Size: 1×96 wells
Incubation Time: 2.5 hours
Sample Type: Serum, Plasma
Sample Size: 10 µL
Alternative Names: Entyvio Assay
This anti-drug antibody(ies) (ADA) kit is a bridging type ELISA for the determination of free antibodies against the drug Vedolizumab in serum and plasma samples. During the first incubation period, ADA in serum or plasma samples are captured by the drug coated to the microtiter wells. After washing away the unbound components from samples, a peroxidase-labelled drug conjugate is added and then incubated. ADA, if present in the sample, will make a bridge, with its identical Fab arms, between the drug coated on the well and the other drug molecule labelled with peroxidase. After a second washing step, the bound enzymatic activity is detected by addition of tetramethylbenzidine (TMB) chromogen-substrate. Finally, the reaction is terminated by adding a stop solution. The positive reaction is expected to be related to the presence of ADA in the sample.
STORAGE AND STABILITY OF THE KIT
The Vedolizumab Antibodies ELISA Assay kit is shipped at ambient temperature and should be stored at 2-8°C. Keep away from heat or direct sun light. The storage and stability of specimen and prepared reagents is stated in the corresponding chapters. The microtiter strips are stable up to the expiry date of the kit in the broken, but tightly closed bag when stored at 2–8°C.