Therapeutic IgG4 ELISA Assay Kit
The Therapeutic IgG4 ELISA Assay Kit is For Research Use Only
Size: 1 x 96 wells
Standard Range: 0.781 ng/mL – 50 ng/mL
Incubation Time: 2 hours 40 minutes
Sample Type: Serum, Plasma
Sample Size: 100 µl
Alternative Name: Immunoglobulin G4, IgG4
Recombinant human monoclonal antibodies have become a major focus of new drug discovery in the Pharmaceutical and Biotech industries, with hundreds of new human monoclonal antibodies currently in preclinical development. Fully human recombinant antibodies have gradually replaced humanized mouse or rat antibodies, with IgG1 and IgG4 as the preferred engineered isotypes. As part of the developmental process, human therapeutic antibody drug candidate molecules must be tested in non-human primate species to assess their pharmacokinetic profile and potential toxicity. Primate and human antibodies are highly homologous, making it difficult to distinguish the human antibody drug candidate from the endogenous primate antibodies in a serum or plasma sample by traditional human IgG-specific immunoassays. Therefore, we developed two highly sensitive ELISAs that distinguish human IgG1 or human IgG4 from rhesus or cynomolgus monkey immunoglobulins. This Recombinant (Therapeutic) Human IgG4 ELISA can be used to accurately quantify human IgG4 in the serum or plasma of rhesus or cynomolgus monkeys without cross reacting with antibodies of those species. This same ELISA can also be used to quantify human IgG4 in the plasma of other preclinical test species (mouse, rat, rabbit, guinea pig, etc.) if desired. This assay is supplied with a recombinant human IgG4 kappa standard. The customer may wish to substitute their own specific drug candidate IgG4 for this standard in the assay.
This is a sandwich enzyme-linked immunosorbent assay (ELISA). The plate is pre-coated with anti-Human IgG Antibody and blocked, ready for the addition of samples and standards. The assay should take approximately 3 hours to run, plus any required sample preparation time.