T4 DNA Ligase Rapid, 600 U/µL
T4 DNA Ligase Rapid is supplied with 5× Rapid Ligation Buffer containing PEG for fast 5–10 minutes ligation, ligation of low-concentration or blunt-end DNA.
T4 DNA Ligase catalyzes the formation of a phosphodiester bond between the terminal 5′ phosphate and the 3′ hydroxyl groups of duplex DNA or RNA. The enzyme efficiently joins blunt and cohesive ends and repairs single stranded nicks in duplex DNA, RNA or DNA–RNA hybrids.
Unit Definition: One unit of T4 DNA Ligase is defined as the amount of enzyme needed to ligate 50% of 100 ng of DNA fragments with cohesive ends in a 50 µl reaction mixture in 1× Ligase buffer incubated at 23°C for 30 minutes. Approximately 22 biotechrabbit cohesive end units are equivalent to one Weiss unit.
This T4 DNA Ligase Rapid, 600 U/µL is manufactured in Germany by biotechrabbit.
