T7 RNA Polymerase, 50 U/µL

T7 RNA Polymerase, 50 U/µL

The T7 RNA Polymerase (50 U/µL) is an extremely promoter-specific, DNA-dependent polymerase from the T7 bacteriophage. The enzyme requires Mg2+ ions and the presence of the T7 promoter in the template DNA to synthesize RNA in the 5´→ 3´ direction. T7 RNA Polymerase (50 U/µL) is for research use only and not to be used for diagnostic procedures.

SKU: BR110120 Category: Tags: ,

T7 RNA Polymerase, 50 U/µL

The combination of the high-specificity binding, which reduces cross-talk with other promoters, and the rigorous purity testing, which confirms absence of other enzymatic activities, ensures that the T7 RNA Polymerase transcribes large amounts of RNA from sequences downstream of the T7 promoter. By using T7 promoters in opposite orientation, double-stranded RNA can be generated. The synthesized RNA is suitable for use in a wide range of applications, including hybridization (e.g., blot, in situ, and microarray), RNA protection, capped RNA and antisense RNA.
Unit Definition: One unit is the enzyme activity which incorporates 1 nmol CMP in acid-precipitable RNA products within one hour at 37°C.

This T7 RNA Polymerase, 50 U/µL is manufactured in Germany by biotechrabbit.

Size 1,000 U | 5,000 U
Component
  • T7 RNA Polymerase – T7 RNA Polymerase, 50 U/µl, in storage buffer containing 50% (v/v) glycerol.
  • T7 RNA Polymerase Reaction Buffer – Optimized 10×T7 RNA Polymerase reaction buffer.
Application Preparation of homogeneously labeled RNA probes for hybridization (e.g., radioactive, biotin, DIG-11-UTP), Generation of mRNA for in vitro translation, gene expression or splicing studies, Production of template for cDNA synthesis, In vitro synthesis of capped RNA transcripts, Synthesis of antisense RNA and dsRNA for RNA interference or gene silencing
Hot Start No
Storage -20°C

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