Anti-Beta2-Glycoprotein-1 IgM ELISA Kit

$280.00

The Anti-β2-glycoprotein 1 IgM (β2-GP1 IgM) test is a semi-quantitative enzyme-linked immunosorbent assay (ELISA) for the detection of the IgM class of autoantibodies specific for β2-GP1 antigen in human serum or sodium citrate, EDTA (K2) and lithium heparin anti-coagulated plasma. It is intended to aid in the assessment of thrombotic risk in patients with autoimmune disease associated with thrombotic disorders such as primary antiphospholipid syndrome or secondary to systemic lupus erythematosus. It is not definitive in isolation. Autoantibody levels represent one parameter in a multicriterion diagnostic process. The Eagle Biosciences Anti-β2-glycoprotein 1 IgM ELISA Assay Kit is for Research Use Only.

Anti-Beta2-Glycoprotein 1 IgM ELISA Kit

Anti-Beta2-Glycoprotein-1 IgM ELISA Kit Developed and Manufactured by Svar Life Science

Size: 1×96 wells
Sensitivity: See Package Insert
Dynamic Range: Cut-off
Incubation Time: 2 hours
Sample Type: Serum, Plasma
Sample Size: 10 µL
For Research Use Only

Controls Included


Sample Collection and Storage:
The Anti-Beta2-Glycoprotein-1 IgM ELISA Kit is recommended for serum/plasma (sodium citrate, EDTA (K2), lithium heparin anticoagulated) samples; do not use lipaemic, haemolysed, icteric or turbid samples. Thoroughly mix thawed samples before assay and avoid repeated freeze/thawing. Do not heat-inactivate samples, this may yield false positive results. Samples may be stored at 1:101 dilution in diluted Sample Diluent 2 for up to 24 hours at 2-8° C, or up to 9 hours at +20° C. For longer storage, store undiluted samples at –20° C.


Assay Background

β2-glycoprotein 1 (β2GP1), or apolipoprotein H, is a plasma glycoprotein that circulates at approximately 200μg/ml. It is associated with plasma lipoproteins, in particular very low-density lipoprotein, and may regulate haemostatic actions occurring on the lipoprotein surface. β2GP1 has been shown to inhibit the intrinsic coagulation pathway via inhibition of Factor XII activation on negatively charged surfaces, and is the dominant antigen for antiphospholipid antibodies (APAs) in patients with anti-phospholipid syndrome.

The presence of APAs such as anti-cardiolipin antibodies is associated with venous and arterial thrombosis, recurrent spontaneous abortions and thrombocytopenia. Anti-cardiolipin antibodies are also present in response to a variety of infections and certain drug treatments. APAs associated with infections are usually transient and not associated with thromboembolic complications; APAs in autoimmune disease are often associated with thromboembolic disease.

Although the mechanism of APA binding is poorly understood, it has been demonstrated that APAs may bind directly to phospholipid or via a protein co-factor, most commonly β2GP1. β2GP1 is often included in anti-cardiolipin assays to ensure the detection of both β2GP1-dependent and β2GP1-independent antibodies. β2GP1-dependent APAs tend to be associated with autoimmune disease; β2GP1-independent APAs tend to be associated with infection. A number of studies have demonstrated that detection of β2GP1 antibodies, in conjunction with anticardiolipin measurement, is important in defining the thrombotic risk associated with APAs.


Related Products

Anti-Beta2-Glycoprotein 1 IgG ELISA Assay Kit
Anti-Beta-2 GP-I Screen ELISA
Anti-Beta 2 GP1 ELISA

Additional Information

Assay Principle


The wells of the microtitre strips are coated with highly purified human β2GP1 antigen. During the first incubation, specific autoantibodies in diluted serum or plasma bind to the antigen-coated surface. The wells are then washed to remove unbound components. In the second incubation, the Conjugate, an enzyme-labeled monoclonal antibody to human IgM, binds any surface-bound autoantibodies. After further washing, specific autoantibodies are traced by incubation with the Substrate. Addition of Stop Solution terminates the reaction, resulting in a colored end-product. The amount of Conjugate bound is measured in absorbance units. In the qualitative protocol, the amount of Conjugate bound by the sample is compared with that bound by the Reference Control. In the semi-quantitative protocol, the concentration of anti-β2GP1 autoantibody can be estimated by interpolation from a dose-response curve based on Standards formulated from a high titer plasma and assigned arbitrary units (U/mL).

Anti-Beta2-Glycoprotein-1 IgM ELISA Kit Standard Curve

Manual

Product Manual