phi29 DNA Polymerase, 10 U/µL

The phi29 DNA Polymerase (10 U/µL) is an exceptionally pure DNA polymerase for demanding applications. The enzyme is purified from a recombinant E. coli strain carrying the phi29 DNA Polymerase gene from bacteriophage phi29. The phi29 DNA Polymerase (10 U/µL) is for research use only and not to be used for diagnostic procedures.

SKU: BR110010 Category: Tags: ,

phi29 DNA Polymerase, 10 U/µL

The enzyme is a highly processive DNA polymerase (up to 70,000 base insertions per binding event) with a powerful strand-displacement activity and a 3’→ 5′ proofreading exonuclease function. phi29 DNA Polymerase proofreading activity acts preferentially on single-stranded DNA or RNA. Therefore, to avoid primer degradation during the DNA synthesis, 3′ modified (protected) primers are highly recommended.
Unit Definition: One unit is defined as the amount of polymerase required to convert 0.5 pmol of dTTP into acid insoluble material in 10 minutes at 30°C.

This phi29 DNA Polymerase, 10 U/µL is manufactured in Germany by biotechrabbit.

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Storage -20°C
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