The Sheep IgG ELISA is designed for the quantitative determination of sheep IgG in complex samples (serum or other biological samples). The Sheep IgG ELISA Assay Kit is for research use only and should not be used in diagnostic procedures.

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The Sheep IgG ELISA is For Research Use Only

Size: 1×96 wells
Sensitivity: 4.69 ng/ml
Dynamic Range: 4.69 – 300 ng/ml
Incubation Time: 2.5 hours
Sample Type: Serum, Biological Fluids
Sample Size: 10 µL
Controls Included

Sample Preparation and Storage
Dilute the HRP conjugate with diluent 1:100 (e. g. 50 µl + 4950 µl diluent). The required amount of conjugate solution should be prepared freshly.

Assay Principle

The determination of sheep IgG is carried out as direct sandwich ELISA. An antibody specific for sheep IgG has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IgG present is bound. After washing away any unbound substances, an enzyme-linked antibody is added. Following a wash, a substrate solution is added to the wells and color develops in proportion to the amount of antibody conjugate. The absorption at 450 nm is proportional to the IgG con­cen­tration.

Related Products

Sheep Haptoglobin ELISA Assay
Feline IgG ELISA Assay
Horse IgG ELISA Assay

Additional Information

Assay Procedure

A. Preparation

  1. Bring all reagents to room temperature
  2. Dilute wash buffer 1:10
  3. Prepare the standard curve from a 1:2-dilution series of standard concentrate with diluent
  4. Dilute samples with diluent
  5. Dilute freshly HRP conjugate 1:100 with diluent

B. Performance

  1. Pipette 100 µl of samples, standards, controls into the wells
  2. Incubate for 1 hour at room temperature with shaking
  3. Wash three times with 300 µl of wash buffer
  4. Add 100 µlof HRP conjugate to each well
  5. Incubate for 1 hour at room temperature with shaking
  6. Wash three times with 300 µlof wash buffer
  7. Dispense 100 µlof TMB substrate solution
  8. Incubate for 30 minutes at room temperature in the dark
  9. Add 100 µl of stop solution
  10. Measure absorption at 450 nm

Package Inserts

Please note: All documents above are for reference use only and should not be used in place of the documents included with this physical product. If digital copies are needed, please contact us.

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