Sheep IgG ELISA
The Sheep IgG ELISA is For Research Use Only
Size: 1×96 wells
Sensitivity: 4.69 ng/ml
Dynamic Range: 4.69 – 300 ng/ml
Incubation Time: 2.5 hours
Sample Type: Serum, Biological Fluids
Sample Size: 10 µL
Sample Preparation and Storage
Dilute the HRP conjugate with diluent 1:100 (e. g. 50 µl + 4950 µl diluent). The required amount of conjugate solution should be prepared freshly.
The determination of sheep IgG is carried out as direct sandwich ELISA. An antibody specific for sheep IgG has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IgG present is bound. After washing away any unbound substances, an enzyme-linked antibody is added. Following a wash, a substrate solution is added to the wells and color develops in proportion to the amount of antibody conjugate. The absorption at 450 nm is proportional to the IgG concentration.