Sf9 HCP ELISA Assay Kit
The Sf9 HCP ELISA Assay Kit is For Research Use Only
Size: 1 × 96 wells
Sensitivity: 3 ng/mL
Dynamic Range: 3 – 243 ng/mL
Incubation Time: 3 hours 35 minutes
Sample Size: 100 μL
Controls Not Included
Assay Principle
The polyclonal antibody in this kit is prepared by using as antigen cleaving HCPs obtained from sf9 cells followed by immunizing sheep to obtain serum and obtaining high-quality antibody by affinity purification method. A double-antibody sandwich method is used for co-incubation by adding calibrators or samples to be tested and HRP-labeled anti-Sf9 HCPs polyclonal antibodies to microtiter plates precoated with anti-Sf9 HCPs polyclonal antibodies; After washing, the color reaction is developed using the added TMB substrate, and finally the enzyme-catalyzed reaction is terminated with stop solution. Absorbance values are measured and read by a microplate reader at 450 nm, which are positively correlated with the concentration of HCPs in the calibrator and sample, and the concentration of Sf9 HCPs in the sample can be calculated from the dose-response curve.
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