Rat IL-4 ELISA Assay

$455.00

The Eagle Biosciences Rat IL-4 ELISA Assay Kit is used as an analytical tool for quantitative determination of Rat Interleukin 4, IL-4 in serum, plasma and cell culture supernatant. The Rat IL-4 ELISA Assay Kit is for research use only and not to be used for diagnostic procedures.

Rat IL-4 ELISA Assay

The Rat IL-4 ELISA Assay is For Research Use Only

Size: 1×96 wells
Sensitivity: 4.46 ng/L
Dynamic Range: 10 – 2000 ng/L
Incubation Time: 1 hour 10 minutes
Sample Type: Serum, Plasma, Cell Culture Supernatant
Sample Size: 40 µl
Alternative Names: Interleukin 4


Assay Principle

The method employs sandwich ELISA technique. Monoclonal antibodies are pre-coated onto microwells. Samples and standards are pipetted into microwells and Rat Interleukin 4, IL-4 present in the sample are bound by the antibodies. Biotin labeled antibody is added and followed by Streptavidin-HRP is pipetted and incubated to form a complex. After washing microwells in order to remove any non-specific binding, the substrate solution (TMB) is added to microwells and color develops proportionally to the amount of Rat Interleukin 4, IL-4 in the sample. Color development is then stopped by addition of stop solution. Absorbance is measured at 450 nm.

Assay Background

The Rat IL-4 ELISA Assay Kit is used for assessing the specific biomarker in samples analytes which may be serum, plasma and cell culture supernatant as validated with the kit. The kit employs a sandwich ELISA technique which leads to a higher specificity and increased sensitivity compared to conventional competitive ELISA kits which employ only one antibody. Double antibodies are used in this kit.


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Package Inserts


Please note: All documents above are for reference use only and should not be used in place of the documents included with this physical product. If digital copies are needed, please contact us.