NOS Colorimetric Non-Enzymatic Assay
The NOS Colorimetric Non-Enzymatic Assay is For Research Use Only
Size: 1 x 96 wells
Sensitivity: 1 pmol/µL or ~0.5 µM
Dynamic Range: 0.5 – 100 µM
Incubation Time: 5 minutes
Sample Type: Biological Fluids
Sample Size: 5 µl
Alternative Name: Nitric Oxide Synthase
Assay Background
This Nitric Oxide Synthase Colorimetric Non-enzymatic Assay allows you to measure total nitric oxide (NO) produced for in vitro experimental systems following conversion of nitrate to nitrite by metallic cadmium. Nitric oxide can be spectrophotometrically assayed by measuring the accumulation of its stable degradation products, nitrate and nitrite. The ratio of these two products in biological fluids, tissue culture media, etc. may vary substantially. Hence, for accurate assessment of the total nitric oxide generated, one must monitor both nitrate and nitrite. An excellent solution to this problem involves the conversion of nitrate to nitrite catalyzed by cadmium. This is then followed by the quantitation of nitrite using the griess reaction.
This kit is suitable for the quantitative determination of total nitric oxide in samples that have high protein concentrations. The cadmium catalyst proves far more robust when in the presence of harsh deproteinating reagents than the alternative, nitrate reductase.
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