Cardiovascular and Oxidative Stress Assay Kit

Mouse SLPI ELISA Kit

$585.00

The Mouse SLPI ELISA Kit is intended for the quantitation of Mouse SLPI concentrations in cell culture supernates, serum and plasma (heparin). The Eagle Biosciences Mouse SLPI ELISA Kit is for research use only and not for diagnostic or therapeutic procedures.

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Mouse SLPI ELISA Kit

The Mouse SLPI ELISA Kit is For Research Use Only

Size: 1×96 wells
Sensitivity: <10pg/ml
Standard Range: 31.2pg/ml-2000pg/ml
Incubation Time: 2 hours 40 minutes
Sample Type: Cell culture supernates, serum and plasma (heparin)
Sample Size: 100 µl
Alternative Names: Antileukoproteinase, Secretory Leukocyte Protease Inhibitor

Assay Principle

The Mouse SLPI Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Mouse SLPI with a 96-well strip plate that is pre-coated with antibody specific for SLPI. The detection antibody is a biotinylated antibody specific for SLPI. The capture antibody is A monoclonal antibody from rat, the detection antibody is a biotinylated detection polyclonal antibody from goat. The kit contains recombinant Mouse SLPI with immunogen: Expression system for standard: NSO, Immunogen sequence: G26-M131. The kit is analytically validated with ready to use reagents. To measure Mouse SLPI, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly proportional to Mouse SLPI in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells’ readings against the standard curve to determine the concentration of Mouse SLPI in the sample.

Related Products

Secretory Leukocyte Peptidase Inhibitor Protein ELISA Assay Kit
Mouse TFF3 ELISA Kit
Mouse PD-L1 ELISA Kit

Additional Information

Assay Background

Antileukoproteinase, also known as secretory leukocyte protease inhibitor (SLPI), is an enzyme that in humans is encoded by the SLPI gene. It is mapped to 2; 2 H3. SLPI is a highly cationic single-chain protein with eight intramolecular disulfide bonds. It is found in large quantities in bronchial, cervical, and nasal mucosa, saliva, and seminal fluids. SLPI inhibits human leukocyte elastase, human cathepsin G, human trypsin, neutrophil elastase, and mast cell chymase. X-ray crystallography has shown that SLPI has two homologous domains of 53 and 54 amino acids, one of which exhibits anti-protease activity (C-terminal domain). The other domain (N-terminal domain) is not known to have any function. This gene encodes a secreted inhibitor which protects epithelial tissues from serine proteases. It is found in various secretions including seminal plasma, cervical mucus, and bronchial secretions, and has affinity for trypsin, leukocyte elastase, and cathepsin G. Its inhibitory effect contributes to the immune response by protecting epithelial surfaces from attack by endogenous proteolytic enzymes; the protein is also thought to have broad-spectrum anti-biotic activity.

Typical Standard Curve

Mouse SLPI ELISA Kit

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