Mouse SAP ELISA Assay


The Mouse SAP ELISA Assay is a highly sensitive two-site ELISA for measuring Serum Amyloid P in biological samples of mice.

Mouse SAP ELISA Assay

The Mouse SAP ELISA Assay is For Research Use Only

Size: 1×96 wells
Sensitivity: 1.845 ng/ml
Dynamic Range: 6.25 ng/ml – 200 ng/ml
Incubation Time: 130 minutes
Sample Type: Plasma, Serum
Sample Size: 100 μL
Alternative Names: Serum amyloid P

Assay Principle

In this Mouse SAP ELISA Assay the SAP present in samples reacts with the anti-SAP antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-SAP antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound SAP. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of serum amyloid P in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of SAP. The quantity of SAP in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.

Blood should be collected by venipuncture. The serum should be separated from the cells after clot formation by centrifugation. For plasma samples, blood should be collected into a container with an anticoagulant and then centrifuged. Care should be taken to minimize hemolysis, excessive hemolysis can impact your results. Assay immediately or aliquot and store samples at -20°C. Avoid repeated freeze thaw cycles.

Related Products

Mouse Serum Amyloid A ELISA Assay Kit
Mouse C3 ELISA Assay

Additional Information

Assay Background

Mouse serum amyloid P (SAP) is a major acute phase protein that circulates as an ~230 kDa glycoprotein. It is composed of 10 identical subunits arranged as two cyclic pentameric discs and contains ~10% carbohydrate. Basal levels of SAP may vary significantly in different inbred mouse strains but SAP is a major acute phase protein in all strains. During acute chemical, physical or inflammatory stimulus, its concentration can increase 50-100 fold within 24-48 hours. Changes can readily be detected in 4-6 hours. Measurement of the concentration of SAP is indicative of the extent and severity of an inflammatory stimulus and can be used to assess various modalities of treatment.

Typical Standard Curve

Mouse SAP ELISA Assay


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