Mouse PM20D1 ELISA Assay Kit

$610.00

The Mouse PM20D1 ELISA Assay Kit is designed for quantification of mouse PM20D1 in serum and plasma samples. The Eagle Biosciences Mouse PM20D1 ELISA Assay Kit is for research use only and not to be used for diagnostic purposes.

SKU: PMD11-K01 Categories: , ,

Mouse PM20D1 ELISA Assay Kit

The Mouse PM20D1 ELISA Assay Kit is For Research Use Only

Size: 1×96 wells
Sensitivity: 0.156ng/mL
Standard Range: 0.312ng/mL-20ng/mL
Incubation Time: 2 hours 15 minutes
Sample Type: Serum and plasma
Sample Size: 100 µl


Assay Principle

The Mouse PM20D1 assay is a quantitative sandwich ELISA. The microplate is pre-coated with a polyclonal antibody specific for mouse PM20D1. Standards and samples are pipetted into the wells and any human PM20D1 present is bound by the immobilized antibody. After washing away any unbound substances, a horseradish peroxidase biotin-labelled polyclonal antibody specific for mouse PM20D1 is added to the wells. After wash step to remove any unbound reagents, streptavidin-HRP conjugate (STP-HRP) is added. After the last wash step, an HRP-substrate solution is added and color develops in proportion to the amount of mouse PM20D1 bound initially. The assay is stopped and the optical density of the wells determined using a microplate reader. Since the increases in absorbance are directly proportional to the amount of captured mouse PM20D1, the unknown sample concentration can be interpolated from a reference curve included in each assay.

Sample Preparation

Serum or plasma sample is generally required at least 100-fold dilution
in the 1×Assay buffer.


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Additional Information

Assay Background


PM20D1 is a bidirectional N-fatty-acyl amino acid synthase/hydrolase that regulates the production of N-fatty-acyl amino acids. These metabolites are endogenous chemical uncouplers of mitochondrial respiration. In an UCP1-independent manner, maybe through interaction with mitochondrial transporters, they promote proton leakage into the mitochondrial matrix. PM20D1 may indirectly regulate the bodily dissipation of chemical energy as heat through thermogenic respiration.

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