Mouse IL-8 ELISA Assay Kit

$455.00

The Eagle Biosciences Mouse IL-8 ELISA Assay Kit is used as an analytical tool for quantitative determination of Mouse Interleukin 8 in serum and plasma. The Mouse IL-8 ELISA Assay Kit is for research use only and not to be used for diagnostic procedures.

SKU: KLM1481 Categories: , ,

Mouse IL-8 ELISA Assay

The Mouse IL-8 ELISA Assay is For Research Use Only
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Size: 1×96 wells
Sensitivity: 17 ng/mL
Dynamic Range: 20 – 320 ng/ml
Incubation Time: 1 hour 10 minutes
Sample Type: Serum, Plasma
Sample Size: 40 µl
Alternative Names: Interleukin 8


Assay Principle

The method employs sandwich ELISA technique. Monoclonal antibodies are pre-coated onto microwells. Samples and standards are pipetted into microwells and Mouse Interleukin 8, IL-8 present in the sample are bound by the antibodies. Then, a HRP (horseradish peroxidase) conjugated secondary antibody is pipetted and incubated to form a complex. After washing microwells in order to remove any non-specific binding, the ready to use substrate solution (TMB) is added to microwells and color develops proportionally to the amount of Mouse Interleukin 8, IL-8 in the sample. Color development is then stopped by addition of stop solution. Absorbance is measured at 450 nm.

Assay Background

The Mouse IL-8 ELISA Assay Kit is used for assessing the specific biomarker in samples analytes which may be serum / plasma as validated with the kit. The kit employs a sandwich ELISA technique which leads to a higher specificity and increased sensitivity compared to conventional competitive ELISA kits which employ only one antibodies. Double antibodies are used in this kit.


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Package Inserts


Please note: All documents above are for reference use only and should not be used in place of the documents included with this physical product. If digital copies are needed, please contact us.