Mouse IL-4 ELISA Assay


The Eagle Biosciences Mouse IL-4 ELISA Assay Kit is used as an analytical tool for quantitative determination of Mouse Interleukin 4, IL-4, in serum, plasma and other biological samples. The Mouse IL-4 ELISA Assay Kit is for research use only and not to be used for diagnostic procedures.

Mouse IL-4 ELISA Assay

The Mouse IL-4 ELISA Assay is For Research Use Only

Size: 1×96 wells
Sensitivity: 0.23 ng/mL
Dynamic Range: 0.5 – 200 ng/ml
Incubation Time: 1 hour 10 minutes
Sample Type: Serum, Plasma, or other biological samples
Sample Size: 40 µl
Alternative Names: Interleukin 4

Assay Principle

The method employs sandwich ELISA technique. Monoclonal antibodies are pre-coated onto microwells. Samples and standards are pipetted into microwells and Mouse Interleukin 4, IL-4 present in the sample are bound by the antibodies. Biotin labeled antibody is added and followed by Streptavidin-HRP is pipetted and incubated to form a complex. After washing microwells in order to remove any non-specific binding, the substrate solution (TMB) is added to microwells and color develops proportionally to the amount of Mouse Interleukin 4, IL-4 in the sample. Color development is then stopped by addition of stop solution. Absorbance is measured at 450 nm.

Assay Background

The Mouse IL-4 ELISA Assay Kit is used for assessing the specific biomarker in samples analytes which may be serum, plasma and cell culture supernatant as validated with the kit. The kit employs a sandwich ELISA technique which leads to a higher specificity and increased sensitivity compared to conventional competitive ELISA kits which employ only one antibody. Double antibodies are used in this kit.

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