Mouse Albumin ELISA


The Eagle Biosciences Mouse Albumin ELISA Assay Kit is designed for the quantitative determination of albumin in mouse urine samples. The Mouse Albumin ELISA Assay Kit is for research use only and should not be used in diagnostic procedures. When blood is filtered in the glomeruli of kidneys, highly molecular substances are held back. Therefore, urine is nearly protein free. In case of kidney damages, small proteins like albumin occur in urine. Albumin there­fore serves as a marker in monitoring kidney damages.


Mouse Albumin ELISA

For Research Use Only

Sensitivity: 0.1 µg/ml
Dynamic Range: 2.5 – 10240 ng/ml
Incubation Time: 2 hours
Sample Type: Urine
Sample Size: 5 µL
Controls Included

Additional Information

Assay Principle

The determination of albumin is carried out as direct competitive ELISA. Mouse albumin has been pre-coated onto a microplate. During incubation the binding of an enzyme-linked anti-albumin(mouse)-antibody to the wells is inhibited by albumin from samples/standards. After washing away any unbound antibody, a substrate solution is added to the wells and color develops in proportion to the amount of antibody conjugate. The absorption at 450 nm is reverse proportional to the albumin con­cen­tration.

Assay Procedure

  1. Prepare all reagents, standard curve and samples as directed in the previous section.
  2. Pipette 50 ml of samples, standards, positive control or diluent (as negative control) into the wells.
  3. Immediately add 50 ml of HRP conjugate to each well.
  4. Mix gently.
  5. Seal wells with adhesive strip and incubate for 1.5 hours at room temperature (with shaking) or 2 h at room temperature without shaking.
  6. Aspirate fluid from wells and wash three times with 300 ml wash buffer.
  7. Dispense 100 ml of TMB substrate solution into each well.
  8. Incubate for 10 minutes at room temperature in the dark.
  9. Add 100 ml of stop solution to each well.
  10. Determine the absorbance within 30 minutes at 450 nm. A reference wavelength of 620 nm/690 nm is recommended.


Product Manual