KL-6 ELISA Assay Kit
The KL-6 ELISA Assay Kit is For Research Use Only
Size: 12×8 wells
Sensitivity: 1.12U/ml
Standard Range: 20-320 U/ml
Incubation Time: 70 minutes
Sample Type: Serum, cell culture supernatant and plasma
Sample Size: 40 ul
Alternative Name: Krebs von den Lungen-6
Assay Background
Krebs von den Lungen 6 (KL-6) is a sensitive marker for diagnosing, monitoring, and predicting the prognoses of interstitial lung diseases (ILDs). Particularly, KL-6 is elevated in the serum of patients with active interstitial pneumonia, making it an ideal biomarker for detection and study of the therapy for the disease. KL-6 is a type of transmembrane mucoprotein-2 secreted by proliferating or damaged type II alveolar epithelial cells. It is specific in judging the function of type II alveolar epithelial cells. The expression of KL-6 is significantly increased when the alveolar epithelium sustains injuries. The KL-6 can be secreted into the bloodstream through the damaged alveolar basement membrane.
Sample Preparation and Storage:
Specimens should be clear and non-hemolyzed. Samples should be run at a number of dilutions to ensure accurate quantitation.
1. Extract as soon as possible after specimen collection as per relevant procedure. The samples should be tested as soon as possible after the extraction. Alternately the extracted samples can be kept in -20°C. Avoid repeated freeze-thaw cycles.
2. Serum- Coagulate at room temperature for 10-20 minutes; centrifuge for 20-min at 2000-3000 rpm. Remove the supernatant. If precipitation appears, recentrifuge.
3. Plasma- Use EDTA or citrate plasma as an anticoagulant, mix for 10-20 minutes; centrifuge for 15-min at 2000-3000 rpm. Remove the supernatant carefully. If precipitation appears, recentrifuge.
4. Urine- Collect urine in a sterile container, centrifuge for 20-min at 2000-3000 rpm. Remove the supernatant. If precipitation appears, recentrifuge.
5. Cell Culture Supernatant- Collect sample in a sterile container. Centrifuge for 20-mins at 2000-3000 rpm. Remove the supernatant carefully. When examining the components within the cell, dilute cell suspension with PBS (pH 7.2-7.4), if cell concentration is greater than 1 million/ml. Damage the cells by repeated freeze-thaw cycles to release intracellular components. Centrifuge for 20-min at 2000-3000 rpm. If precipitation appears, centrifuge again.
6. Tissue Samples- Rinse tissues in PBS (pH 7.4) to remove excess blood thoroughly and weigh before homogenization. Mince tissues and homogenize them in PBS (pH7.4) with a glass homogenizer on ice. Thaw at 2-8°C or freeze at -20°C. Centrifuge at 2000-3000 RPM for approximately 20 minutes and collect the supernatant carefully.
Note: Grossly hemolyzed samples are not suitable for use in this assay.
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