Inorganic Pyrophosphatase ELISA Detection Kit
The Inorganic Pyrophosphatase ELISA Detection Kit is For Research Use Only
Size: 8 x 12 wells
Sensitivity: 0.25 ng/mL
Dynamic Range: 0.25 – 16 ng/mL
Sample Size: 100 uL
Incubation Time: 3 hours 15 minutes
Sample Type: various biological products
Assay Background
Inorganic pyrophosphatase (PPase) can catalyze the conversion of one molecule of pyrophosphate into two molecules of phosphate ions. PPase can avoid inhibiting the reaction system due to the accumulation of inorganic pyrophosphate in nucleic acid amplification experiments. In the production of mRNA vaccine products, PPase is added to increase yield. Therefore, detection of PPase residues is required for mRNA vaccine products.
Assay Principle
This kit uses double-antibody sandwich enzyme-linked immunosorbent assay (ELISA) method. Add PPase standard and test samples to the microtiter plate precoated with anti-PPase antibody, then add diluted biotin-labeled PPase detection antibody, finally add streptavidin-HRP to form the antibody + antigen + antibody-Biotin + SA-HRP complex, wash the plate and add TMB chromogenic solution for color development. TMB is converted from colorless to blue under the catalysis of HRP enzyme and finally to yellow under the action of stop solution. The shade of yellow is positively correlated with the amount of PPase detected in the sample.
Storage Conditions and Shelf Life
The product should be stored and transported at 2-8℃; the shelf life is 12 months.
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