iLite ADCC Effector FcγRIIIa (V) Assay Ready Cells


The iLite® ADCC Effector FcγRIIIa (V) Assay Ready Cells are designed for the specific detection of drug potency in serum/plasma as well as neutralizing antibodies. They utilize an assay technique called reporter gene assay ( RPG ) to analyze serum/plasma samples. The reporter genes utlized are encoded with a bioluminescent luciferase (Firefly luciferase). Different levels of luminescence detected from each reporter gene cell indicates different levels of expression. The Eagle Biosciences iLite® ADCC Effector FcγRIIIa (V) Assay Ready Cells are for research use only and not for use in diagnostic procedures.

iLite ADCC Effector FcγRIIIa (V) Assay Ready Cells

iLite ADCC Effector FcγRIIIa (V) Assay Ready Cells Developed and Manufactured by Svar Life Science

The Eagle Biosciences iLite ADCC Effector FcγRIIIa (V) Assay Ready Cells are:

  • included as part of the iLite Anti-CD20 ADCC Activity Set
  • can be used together with matched iLite ADCC Target CD20 (+) and iLite ADCC Target CD20 (-) Assay Ready Cells for the quantification ADCC activity.

For Research Use Only

Key benefits of iLite ADCC Activity Assays

  • Unparalleled sensitivity
  • High serum tolerance
  • Normalization read-out included
  • Negative control available for screening of unspecific activity
  • Easy to use – no culturing required on target or effector cells


Antibody-dependent cell-mediated cytotoxicity (ADCC) is a mechanism whereby pathogenic cells are lysed by lymphocytes, most often Natural Killer (NK) cells. The mechanism involves binding of antibodies to surface antigens on the pathogen. Crosslinking of these antibodies to NK cells through the binding of the Fc-portion to Fc receptors on the NK cells leads to activation of the NK cell and formation of an immune synapse with the pathogenic cell. The NK cell releases cytotoxic granules containing granzymes and perforin into the synapse, leading to apoptosis of the targeted cell .

The idea of employing ADCC to destroy dysfunctional cells by treating patients with antibodies which induce this mechanism has existed since the discovery of the ADCC mechanism. The first monoclonal antibody for treating cancer to be FDA approved was Rituximab which in part utilizes the ADCC mechanism to destroy cancer cells expressing CD20. Induction of ADCC through monoclonal antibodies is also utilized in treating autoimmune diseases.

Products Related to iLite Effector V Assay Ready Cells

iLite ADCC Target mTNF-alpha (-)
iLite ADCC Target mTNF-alpha (+)
iLite ADCC Target EGFR (-)

Additional Information


iLite® ADCC Effector (V) Assay Ready Cells are based on a human T lymphocyte cell line, Jurkat (ATCC #TIB-152), and have been genetically engineered and optimized to express high levels of the low affinity Fc receptor FcγIIIa (CD16), and the Firefly Luciferase (FL) reporter gene under the control of a proprietary chimeric promoter. The FcγIIIa receptor responds to ligation of the Fc moiety of an antibody bound to the specific antigen on target cells by activation of the FL reporter gene.

Normalization of cell counts, serum matrix effects or lysis of the effector cells by the target cells is obtained by a second reporter gene, a NanoLuc Luciferase reporter gene construct, under control of a constitutive promotor.

Discover the Benefits of iLite


Package Inserts

Please note: All documents above are for reference use only and should not be used in place of the documents included with this physical product. If digital copies are needed, please contact us.

Product Citations