IL-4 Human ELISA Assay


The Eagle Biosciences Human IL-4 ELISA Assay Kit is specifically designed for the accurate quantitation of human IL-4 from cell culture supernatant, serum, plasma or other bodily fluids. It is ready-to-use, accurate, and sensitive. The Human IL-4 ELISA Assay Kit is for research use only and not to be used for diagnostic procedures.

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IL-4 Human ELISA Assay

The IL-4 Human ELISA Assay is For Research Use Only

Size: 1×96 wells
Sensitivity: 62.5 pg/mL
Dynamic Range: 31.3 – 2000 pg/ml
Incubation Time: 1.5 hours
Sample Type: Serum, Plasma, Cell Culture Supernatants, and other biological fluids
Sample Size: 100 µl
Alternative Names: Interleukin 4

Assay Background

Interleukin-4, abbreviated IL-4, is a cytokine that induces differentiation of naïve helper T cells (Th0 cells) to Th2 cells. Upon activation by IL-4, Th2 cells subsequently produce additional IL-4. It has many biological roles, including the stimulation of activated B-cell and T-cell proliferation, and the differentiation of CD4+ T-cells into Th2 cells. It is a key regulator in humeral and adaptive immunity. IL-4 induces B-cell class switching to Ice, and up-regulates MHC class II production. Overproduction of IL-4 is associated with allergies.

Specimen Collection and Handling

Specimens should be clear and non-hemolyzed. Samples should be run at a number of dilutions to ensure accurate quantitation.

Cell Culture Supernatant: If necessary, centrifuge to remove debris prior to analysis. Samples can be stored at temperature < -20°C. Avoid repeated freeze/thaw cycles.

Serum: Use a serum separator tube and allow clotting for 30 minutes, then centrifuge for 10 minutes at 1000 x g. Remove serum layer and assay immediately or store serum samples at temperature <-20°C. Avoid repeated freeze/thaw cycles.

Plasma: Collect blood sample in a citrate, heparin or EDTA containing tube. Centrifuge for 10 minutes at 1000 x g within 30 minutes of collection. Assay immediately or store plasma samples at temperature < -20°C. Avoid repeated freeze/thaw cycles.

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