IL-4 Human ELISA Assay


The Eagle Biosciences Human IL-4 ELISA Assay Kit is specifically designed for the accurate quantitation of human IL-4 from cell culture supernatant, serum, plasma or other bodily fluids. It is ready-to-use, accurate, and sensitive. The Human IL-4 ELISA Assay Kit is for research use only and not to be used for diagnostic procedures.

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IL-4 Human ELISA Assay

The IL-4 Human ELISA Assay is For Research Use Only

Size: 1×96 wells
Sensitivity: 62.5 pg/mL
Dynamic Range: 31.3 – 2000 pg/ml
Incubation Time: 1.5 hours
Sample Type: Serum, Plasma, Cell Culture Supernatants, and other biological fluids
Sample Size: 100 µl
Alternative Names: Interleukin 4

Assay Background

Interleukin-4, abbreviated IL-4, is a cytokine that induces differentiation of naïve helper T cells (Th0 cells) to Th2 cells. Upon activation by IL-4, Th2 cells subsequently produce additional IL-4. It has many biological roles, including the stimulation of activated B-cell and T-cell proliferation, and the differentiation of CD4+ T-cells into Th2 cells. It is a key regulator in humeral and adaptive immunity. IL-4 induces B-cell class switching to Ice, and up-regulates MHC class II production. Overproduction of IL-4 is associated with allergies.

Specimen Collection and Handling

Specimens should be clear and non-hemolyzed. Samples should be run at a number of dilutions to ensure accurate quantitation.

Cell Culture Supernatant: If necessary, centrifuge to remove debris prior to analysis. Samples can be stored at temperature < -20°C. Avoid repeated freeze/thaw cycles.

Serum: Use a serum separator tube and allow clotting for 30 minutes, then centrifuge for 10 minutes at 1000 x g. Remove serum layer and assay immediately or store serum samples at temperature <-20°C. Avoid repeated freeze/thaw cycles.

Plasma: Collect blood sample in a citrate, heparin or EDTA containing tube. Centrifuge for 10 minutes at 1000 x g within 30 minutes of collection. Assay immediately or store plasma samples at temperature < -20°C. Avoid repeated freeze/thaw cycles.

Related Products

Human IL-2 ELISA Assay
Human IL-9 ELISA Assay
Human IL-12 p40 ELISA Assay Kit

Package Inserts

Please note: All documents above are for reference use only and should not be used in place of the documents included with this physical product. If digital copies are needed, please contact us.

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